Prevalence and characterization of Salmonella serovars in retail meats of marketplace in Shaanxi, China

A total of 764 retail meat including 515 chicken, 91 pork, 78 beef and 80 lamb samples were collected in Shaanxi Province of China in 2007–2008 to determine the prevalence of Salmonella. The isolates were characterized using serotyping, antimicrobial susceptibility testing, and the presence of bla C...

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Bibliographic Details
Published in:International journal of food microbiology 2010-06, Vol.141 (1), p.63-72
Main Authors: Yang, Baowei, Qu, Dong, Zhang, Xiuli, Shen, Jinling, Cui, Shenghui, Shi, Ying, Xi, Meili, Sheng, Min, Zhi, Shuai, Meng, Jianghong
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents
antibiotic resistance
bacterial contamination
Base Sequence
beta-Lactamases - genetics
Biological and medical sciences
Cattle
Characterization
Chickens
China
Commerce
Drug Resistance, Multiple, Bacterial
Electrophoresis, Gel, Pulsed-Field - methods
food analysis
food contamination
Food industries
Food Microbiology
food pathogens
Fundamental and applied biological sciences. Psychology
Integrons
meat
Meat - microbiology
Meat and meat product industries
meat quality
Prevalence
retail marketing
Retail meats
Salmonella
Salmonella - classification
Salmonella - genetics
Salmonella - isolation & purification
serotypes
Serotyping - methods
Sheep
Swine
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Summary:A total of 764 retail meat including 515 chicken, 91 pork, 78 beef and 80 lamb samples were collected in Shaanxi Province of China in 2007–2008 to determine the prevalence of Salmonella. The isolates were characterized using serotyping, antimicrobial susceptibility testing, and the presence of bla CMY-2 and bla TEM and class I integrons. Selective serovars were further subtyped using PFGE. Approximately 54% (276) of chicken, 31% (28) of pork, 17% (13) of beef and 20% (16) of lamb samples were positive of Salmonella. Among 24 serovars identified, Enteritidis (31.5%) was most common, followed by Typhimurium (13.4%), Shubra (10.0%), Indiana (9.7%), Derby (9.5%) and Djugu (7.0%). Nearly 80% of the isolates (283) were resistant to at least one antimicrobial, and 53% (191) to more than three antimicrobials. Resistance was most frequently observed to sulfamethoxazole (67%), to trimethoprim/sulfamethoxazole (58%) and to tetracycline (56%). Furthermore, many isolates were resistant to nalidixic acid (35%), ciprofloxacin (21%) and ceftriaxone (16%). Most isolates of Shubra (89%) and Indiana (88%) were resistant to ≥ 9 antimicrobials, compared to only 11% of Enteritidis and 9% of Infantis that showed similar resistance. Class I integrons were detected in 10% of the isolates, and contained aadA, tetR, dhfr, bla PSE-1, bla DHA-1 and bla VEB-1 gene cassettes alone or various combinations. Ceftriaxone- and/or cefoperazone-resistant isolates ( n = 62) carried bla TEM (51.6%) and/or bla CMY-2 (56.5%). A total of 116 PFGE patterns were generated among 210 selected isolates. Our findings indicated that Salmonella contamination was common in retail meats, and that the Salmonella isolates were phenotypically and genetically diverse. Additionally, many Salmonella isolates were resistant to multiple antimicrobials.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2010.04.015