Survey of natural infection by Leishmania in sand fly species collected in southeastern Brazil

In this study, we sought to identify sand fly vectors of the Leishmania species that circulate in distinct eco-epidemiological disease-endemic rural areas within the Espírito Santo State in southeastern Brazil. PCR amplification of a conserved region of the minicircle kDNA was used to estimate infec...

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Bibliographic Details
Published in:Transactions of the Royal Society of Tropical Medicine and Hygiene 2010-07, Vol.104 (7), p.461-466
Main Authors: Rocha, Leonardo S., Falqueto, Aloísio, dos Santos, Claudiney B., Ferreira, Adelson L., da Graça, Grazielle C., Grimaldi, Gabriel, Cupolillo, Elisa
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brazil
Disease transmission
Female
General aspects
Human protozoal diseases
Infectious diseases
Leishmania braziliensis
Leishmania braziliensis - genetics
Leishmania braziliensis - growth & development
Leishmania braziliensis - isolation & purification
Leishmaniasis
Leshmaniasis
Lutzomyia ferreirana
Lutzomyia fischeri
Medical sciences
natural infection
Parasitic diseases
Polymerase Chain Reaction
Polymorphism, Genetic
Population Density
Protozoal diseases
Psychodidae - classification
Psychodidae - parasitology
Sand flies
Sequence Analysis, DNA
Species Specificity
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Summary:In this study, we sought to identify sand fly vectors of the Leishmania species that circulate in distinct eco-epidemiological disease-endemic rural areas within the Espírito Santo State in southeastern Brazil. PCR amplification of a conserved region of the minicircle kDNA was used to estimate infection rates in field-captured, peridomestic female sand flies. Only 13 of the 1689 female sand fly specimens (0.77%) actually contained Leishmania DNA. Leishmania braziliensis infections were found in Lutzomyia intermedia and Lu. whitmani, and, for the first time, in Lu. fischeri and Lu. ferreirana. Interestingly, the high rate of genetic polymorphism of the L. braziliensis parasites in one of the disease-endemic areas that were studied may reflect specific transmission cycles involving different sand fly vectors.
ISSN:0035-9203
1878-3503
DOI:10.1016/j.trstmh.2010.02.005