novel catabolic activity of Pseudomonas veronii in biotransformation of pentachlorophenol

Pseudomonas veronii PH-05, a bacterial strain capable of transforming pentachlorophenol (PCP) to a metabolic intermediate, was isolated by selective enrichment of soil samples from a timber storage yard. Strain PH-05 was shown to be able to grow using PCP as the sole source of carbon and energy. GC-...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2003-08, Vol.62 (2/3), p.284-290
Main Authors: Nam, I.H, Chang, Y.S, Hong, H.B, Lee, Y.E
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Biotransformation
carbon
Catechols - pharmacokinetics
Culture Media
energy
Environmental Pollutants - pharmacokinetics
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
growth retardation
hydroquinone
Kinetics
Metabolism
Microbiology
Mission oriented research
Molecular microbiology
Observations
Pentachlorophenol
Pentachlorophenol - pharmacokinetics
Physiology and metabolism
Pseudomonas
Pseudomonas - growth & development
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Pseudomonas veronii
Soil Microbiology
soil sampling
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Summary:Pseudomonas veronii PH-05, a bacterial strain capable of transforming pentachlorophenol (PCP) to a metabolic intermediate, was isolated by selective enrichment of soil samples from a timber storage yard. Strain PH-05 was shown to be able to grow using PCP as the sole source of carbon and energy. GC-MS analysis showed that the metabolic intermediate was tetrachlorocatechol, which inhibited the growth of this strain. The formation of tetrachlorocatechol during biotransformation was monitored, and its inhibitory effect on growth of strain PH-05 was analyzed at a range of concentrations. The catabolic activity of the isolated strain differs from that of other PCP-degrading bacteria, which metabolize PCP through a chlorinated hydroquinone intermediate.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-003-1255-1