Oxidative stress defense in human-skin-derived mesenchymal stem cells versus human keratinocytes: Different mechanisms of protection and cell selection

Stem cells are undifferentiated cells with the capacity for self-renewal and differentiation. Here we have determined the susceptibility to oxidative stress of isolated mesenchymal stem cells from human skin (S-MSCs) in comparison with keratinocytes, which are differentiated cells of the same lineag...

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Bibliographic Details
Published in:Free radical biology & medicine 2010-09, Vol.49 (5), p.830-838
Main Authors: Orciani, M., Gorbi, S., Benedetti, M., Di Benedetto, G., Mattioli-Belmonte, M., Regoli, F., Di Primio, R.
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Cell Separation
Cell Survival - drug effects
Cells, Cultured
Cytoprotection - physiology
Differentiation
Free radicals
Humans
Hydrogen Peroxide - pharmacology
Immunity, Cellular - drug effects
Immunity, Cellular - physiology
Keratinocytes - drug effects
Keratinocytes - immunology
Keratinocytes - metabolism
Keratinocytes - physiology
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - immunology
Mesenchymal Stromal Cells - physiology
Oxidative stress
Oxidative Stress - drug effects
Oxidative Stress - immunology
Skin
Skin - cytology
Skin - immunology
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Summary:Stem cells are undifferentiated cells with the capacity for self-renewal and differentiation. Here we have determined the susceptibility to oxidative stress of isolated mesenchymal stem cells from human skin (S-MSCs) in comparison with keratinocytes, which are differentiated cells of the same lineage. To induce pro-oxidant conditions, S-MSCs and keratinocytes were exposed to 0.5 mM H 2O 2 for 2 h, with oxidative effects analyzed after 4, 12, 24, and 48 h of recovery, in terms of cell growth, vitality, apoptosis, DNA damage, variations in individual antioxidant defense and total oxyradical scavenging capacity toward peroxyl and hydroxyl radicals. The data indicate different abilities across these two cell types to counteract this oxidative stress, which reflects stress that would normally be experienced by these cells under basal conditions. Human keratinocytes seem to have much greater antioxidant defense to counteract the oxidative injury to which they are continuously exposed in the skin. The S-MSCs are surrounded by a complex microenvironment that protects them from external insults, and so they do not have a particularly efficient defense system, and they were generally less responsive to enhanced pro-oxidant challenge. S-MSCs seem particularly prone to apoptotic events, which might thus represent their primary defense mechanism against stress.
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2010.06.007