Enhancement of taxol production and release in Taxus chinensis cell cultures by ultrasound, methyl jasmonate and in situ solvent extraction

This study evaluates the use of a novel mechanical stimulus, ultrasound (US), and a putative chemical elicitor, methyl jasmonate (MJ), combined with in situ solvent extraction (two-phase culture), to enhance taxol production by Taxus chinensis cells in suspension culture. The volumetric taxol yield...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2003-08, Vol.62 (2/3), p.151-155
Main Authors: Wu, J, Lin, L
Format: Article
Language:English
Subjects:
Acetates - pharmacology
Analysis
biochemical pathways
Biological and medical sciences
Biosynthesis
Biotechnology
Cell culture
Conifers
Cyclopentanes - pharmacology
Dibutyl Phthalate
elicitors
Enzymatic activity
enzyme activity
Eukaryotic cell cultures
Extraction processes
Fundamental and applied biological sciences. Psychology
Jasmonates
Methods. Procedures. Technologies
methyl jasmonate
Miscellaneous
Molecular microbiology
Oxylipins
paclitaxel
Paclitaxel - biosynthesis
Paclitaxel - isolation & purification
Plant cells and fungal cells
Plant Growth Regulators - pharmacology
Solvent extraction
Solvent extraction processes
Solvents
Taxus
Taxus - drug effects
Taxus - growth & development
Taxus - metabolism
Taxus chinensis
Ultrasonic imaging
ultrasonic treatment
Ultrasonics
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Summary:This study evaluates the use of a novel mechanical stimulus, ultrasound (US), and a putative chemical elicitor, methyl jasmonate (MJ), combined with in situ solvent extraction (two-phase culture), to enhance taxol production by Taxus chinensis cells in suspension culture. The volumetric taxol yield was increased 1.5- to 1.8-fold with 2 min US treatment once or twice during a 4-week culture period, about 5-fold with 60-120 micromolar MJ, and 7- to 9-fold by in situ solvent extraction of taxol with dibutyl phthalate (DBP) (11% v/v). The percent of extracellular taxol or taxol release was also significantly increased. The combined use of US (day 5 or 9) and MJ treatment (day 7) resulted in taxol yields 20-50% higher than each of the treatments used alone. The most favorable strategy for taxol production was the application of US or MJ treatment, followed by in situ solvent extraction, giving rise to a taxol yield of 33-35 mg/l, about 17-fold higher than the control, at 1.9 mg/l. It was found that the organic solvent DBP, as well as US and MJ, stimulated the enzyme activity of secondary metabolic pathways, which was partially responsible for the enhanced taxol production.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-003-1275-x