Effects of intraventricular infusion of vascular endothelial growth factor on cerebral blood flow, edema, and infarct volume

Therapeutic cerebral angiogenesis, utilizing angiogenic factors to enhance collateral vessel formation within the central nervous system, is a potential method for cerebral revascularization. A prior dose-response study determined that intracerebroventricular infusion of vascular endothelial growth...

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Bibliographic Details
Published in:Acta neurochirurgica 2003, Vol.145 (1), p.49-53
Main Authors: HARRIGAN, M. R, ENNIS, S. R, SULLIVAN, S. E, KEEP, R. F
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain Edema - etiology
Brain Edema - physiopathology
Brain Edema - prevention & control
Brain Infarction - etiology
Brain Infarction - physiopathology
Brain Infarction - prevention & control
Cerebrovascular Circulation - drug effects
Cerebrovascular Circulation - physiology
Disease Models, Animal
Endothelial Growth Factors - administration & dosage
Endothelial Growth Factors - pharmacology
Endothelial Growth Factors - therapeutic use
Humans
Infarction, Middle Cerebral Artery - complications
Infarction, Middle Cerebral Artery - drug therapy
Infarction, Middle Cerebral Artery - physiopathology
Infusions, Parenteral
Injections, Intraventricular
Intercellular Signaling Peptides and Proteins - administration & dosage
Intercellular Signaling Peptides and Proteins - pharmacology
Intercellular Signaling Peptides and Proteins - therapeutic use
Lymphokines - administration & dosage
Lymphokines - pharmacology
Lymphokines - therapeutic use
Medical sciences
Neovascularization, Physiologic - drug effects
Neovascularization, Physiologic - physiology
Neurology
Rats
Rats, Sprague-Dawley
Severity of Illness Index
Vascular diseases and vascular malformations of the nervous system
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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Summary:Therapeutic cerebral angiogenesis, utilizing angiogenic factors to enhance collateral vessel formation within the central nervous system, is a potential method for cerebral revascularization. A prior dose-response study determined that intracerebroventricular infusion of vascular endothelial growth factor (VEGF) increases vascular density with minimal associated brain edema at a concentration of 5 microg/ml. The purpose of this study was to assess effects of intracerebroventricular infusion of VEGF (5 microg/ml) on cerebral blood flow, infarct volume, and brain edema after ischemia. Recombinant human VEGF(165) was infused into the right lateral ventricle of rats with an osmotic minipump at a rate of 1 microl/hr for 7 days. Control animals received vehicle only. Ischemia was produced by transient (2 hours) middle cerebral artery occlusion (MCAO). After MCAO, cerebral blood flow was determined with the indicator fractionation technique: infarct volume was assessed with 2,3,5-triphenlytetrazolium chloride staining, and brain edema was determined by measuring brain water content. Cerebral blood flow was not significantly different in animals treated with VEGF compared to controls. There was a significant reduction in total infarct volume after temporary MCAO in VEGF-treated animals compared to controls (163+/-37 mm(3) vs. 309+/-54 mm(3), P
ISSN:0001-6268
0942-0940
DOI:10.1007/s00701-002-1035-1