Stage-specific effects of osmolarity of a culture medium on development of pig oocytes and miniature pig somatic cell nuclear transfer embryos activated by ultrasound treatment

Whether high osmolarity of a culture medium at the early culture stage affects the development of pig oocytes and miniature pig somatic cell nuclear transfer (SCNT) embryos activated by ultrasound was examined. When oocytes were cultured in modified porcine zygote medium-3 (mPZM-3) with increased Na...

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Bibliographic Details
Published in:Animal science journal 2010-08, Vol.81 (4), p.453-460
Main Authors: Mizobe, Yamato, Kurino, Saori, Sata, Yoshiaki, Mori, Hironori, Yoshida, Mitsutoshi, Miyoshi, Kazuchika
Format: Article
Language:English
Subjects:
Animal reproduction
Animal sciences
Animals
Cell culture
Culture Media
Embryology
Hogs
in vitro development
NaCl concentration
nuclear transfer
Nuclear Transfer Techniques
Oocytes - growth & development
Osmolar Concentration
osmolarity
Swine - physiology
Swine, Miniature - physiology
Ultrasonic technology
Ultrasonics
ultrasound
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Summary:Whether high osmolarity of a culture medium at the early culture stage affects the development of pig oocytes and miniature pig somatic cell nuclear transfer (SCNT) embryos activated by ultrasound was examined. When oocytes were cultured in modified porcine zygote medium-3 (mPZM-3) with increased NaCl to 138 mmol/L (mPZM-3+NaCl; 326 mOsm) or 50 mmol/L sucrose (mPZM-3+sucrose; 318 mOsm) for the first 2 days and then cultured in normal mPZM-3 (273 mOsm) for 5 days, the cleavage and blastocyst formation rates were significantly (P < 0.05) higher than those of oocytes cultured in mPZM-3 for 7 days. The cleavage and blastocyst formation rates of SCNT embryos cultured in mPZM-3+NaCl for the first 2 days and then cultured in mPZM-3 for 5 days were also significantly (P < 0.05) higher than those of embryos cultured in mPZM-3 for 7 days. These results showed that the high osmolarity of a culture medium induced by increasing NaCl concentration during the first 2 days improves the development of pig oocytes and miniature pig SCNT embryos activated by ultrasound.
ISSN:1344-3941
1740-0929
DOI:10.1111/j.1740-0929.2010.00758.x