Comparative 1H NMR studies of saturation transfer in copolymer gels and mouse lenses

Saturation transfer in cross‐linked copolymer gels and excised intact and perforating trauma‐induced cataract mouse lenses (4‐ or 8‐week‐old) were studied using intermolecular cross‐relaxation rates (1/TIS(H2O); 1/TIS), monitored with f2‐irradiation at −8.79, −4.00, and 7.13 ppm (γH2/2π ∼ 69 Hz). [1...

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Published in:NMR in biomedicine 2010-07, Vol.23 (6), p.584-591
Main Authors: Nakamura, Koji, Sogami, Masaru, Era, Seiichi, Matsushima, Shigeru, Kinosada, Yasutomi
Format: Article
Language:English
Subjects:
Animals
cataractous mouse lens
Gels - chemistry
hydrophilic copolymer gels
hydrophobic copolymer gels
intact mouse lens
intermolecular cross-relaxation rate
Lens, Crystalline - chemistry
Mice
Nuclear Magnetic Resonance, Biomolecular - methods
Polymers - chemistry
spin-lattice relaxation rate
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Summary:Saturation transfer in cross‐linked copolymer gels and excised intact and perforating trauma‐induced cataract mouse lenses (4‐ or 8‐week‐old) were studied using intermolecular cross‐relaxation rates (1/TIS(H2O); 1/TIS), monitored with f2‐irradiation at −8.79, −4.00, and 7.13 ppm (γH2/2π ∼ 69 Hz). [1] The 1/TIS(7.13 ppm) vs dry weight [W (%)] profiles for hydrophilic copolymer gels were far steeper than those for hydrophobic copolymer gels, indicating the participation of an amount of bound water and a number of copolymer hydroxyl groups in the saturation transfer process. In contrast, the 1/TIS(−8.79 ppm) vs W (%) profiles exhibited little difference between the hydrophilic and hydrophobic copolymer gels, indicating the major participation of molecular rigidity, i.e. W (%) in the saturation transfer process. [2] The 1/TIS(7.13 ppm) values for cataractous mouse lenses were larger than those for intact lenses, indicating the formation of large, immobile lens protein associates or aggregates containing a sufficient amount of bound water for the saturation transfer. [3] The 1/TIS(7.13 ppm) vs W (%) profiles for the hydrophilic copolymer gels exhibited similar characteristics to the intact and cataractous mouse lenses with regard to the saturation transfer process. Copyright © 2010 John Wiley & Sons, Ltd. Saturation transfer phenomena from irradiated protein protons to observed water protons were studied using intermolecular cross‐relaxation rates (1/TIS(H2O)), monitored with f2‐irradiation at −8.79, −4.00 and 7.13 ppm (500 MHz 1H‐MRS). The comparison is performed in both synthetic cross‐linked copolymer gels and mouse lenses. The 1/TIS(H2O) vs dry weight (W (%) profiles may be useful for searching the physical state of water for copolymer gels as well as tissue characterization for the excised and/or living tissues.
ISSN:0952-3480
1099-1492
DOI:10.1002/nbm.1499