Performance of BD Phoenix in Identification and Susceptibility Testing of Streptococcus pneumoniae

The performance of the BD Phoenix SMIC/ID8 Streptococcus Panel (BDSP) for identification (ID) and susceptibility testing of Streptococcus pneumoniae (S. pneumoniae) was compared to the Clinical and Laboratory Standards Institute (CSLI) method. Mutations of penicillin-binding protein (PBP) genes were...

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Bibliographic Details
Published in:Laboratory medicine 2010-07, Vol.41 (7), p.403-409
Main Authors: Nakamura, Tatsuya, Sato, Mayumi, Shimizu, Chihiro, Okuda, Kazuyuki, Nakata, Chiyo, Fujimoto, Hiroko, Ookura, Hiroe, Takahashi, Hakuo
Format: Article
Language:English
Subjects:
Comparative studies
Diagnostic tests
Infectious diseases
Microbiology
Penicillins
Pneumonia
Protein binding
Streptococcus
Streptococcus infections
Streptococcus pneumoniae
Testing laboratories
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Summary:The performance of the BD Phoenix SMIC/ID8 Streptococcus Panel (BDSP) for identification (ID) and susceptibility testing of Streptococcus pneumoniae (S. pneumoniae) was compared to the Clinical and Laboratory Standards Institute (CSLI) method. Mutations of penicillin-binding protein (PBP) genes were detected by polymerase chain reaction (PCR), and the minimum inhibitory concentrations (MIC) values obtained from BDSP were compared with the PCR results. To identify bacterial isolates, the concordance rate to those with autolysin gene was 97.1%. The MIC50 and MIC90 with the BDSP and CLSI method were in close agreement in non-mutants and single PBP mutants. However, when the number of PBP mutations increased, MICs increased further and demonstrated resistance to penicillin (PEN) in particular. Thereby, MICs by BDSP were higher than those by the CLSI method (P
ISSN:0007-5027
1943-7730
DOI:10.1309/LMVYYBTF2BTJQKX0