Enzyme-linked immunosorbent assays for the synthetic steroid gestrinone

Gestrinone is a synthetic steroid hormone with anti-estrogenic and anti-progesterone properties. It is used to treat endometriosis, shrink uterine fibroids and reduce menorrhagia; besides, it has been investigated for use as contraceptive. Also, due to its anabolic effects, it has been included in t...

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Bibliographic Details
Published in:Talanta (Oxford) 2010-09, Vol.82 (4), p.1581-1587
Main Authors: Brun, Eva M., Hernández-Albors, Alejandro, Ventura, Rosa, Puchades, Rosa, Maquieira, Ángel
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid
Cross Reactions
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Exact sciences and technology
Gestrinone - analysis
Gestrinone determination
Humans
Limit of Detection
Miscellaneous
Other chromatographic methods
Spectrometric and optical methods
Synthetic steroids
Tandem Mass Spectrometry
Urine
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Summary:Gestrinone is a synthetic steroid hormone with anti-estrogenic and anti-progesterone properties. It is used to treat endometriosis, shrink uterine fibroids and reduce menorrhagia; besides, it has been investigated for use as contraceptive. Also, due to its anabolic effects, it has been included in the banned list of performance enhanced drugs in sport. Polyclonal antibodies raised against bovine serum albumin coupled to gestrinone 3-carboxymethyloxime (3OCMO-G) were used to develop two highly sensitive and specific enzyme-linked immunosorbent assays for gestrinone. One of them, based on direct format, shows a detection limit (LD) of 0.09 ± 0.03 ng L −1. The second assay, hapten-protein coating format, can detect until (LD) 0.14 ± 0.05 ng L −1. Both immunoassays were also highly specific, showing negligible or no cross-reactivity to other anabolic steroids. The developed ELISAs detected lower amounts of gestrinone than those determined by the reference chromatographic HPLC/MS/MS methods. The direct format was applied to quantify this steroid in spiked human urine without sample pre-treatment, with recovery values between 76 and 122%.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2010.07.067