Inhibitory effects of Hoelen extract on melanogenesis in B16/F1 melanoma cells

Melanin synthesis is regulated by melanogenic proteins, such as tyrosinase, tyrosinase-related protein 1 (TRP-1) and TRP-2. The effects of Hoelen extract on melanogenesis were investigated in B16Fl murine melanoma cells. Specifically, tyrosinase activity, cell viability and melanin content were assa...

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Bibliographic Details
Published in:Phytotherapy research 2010-09, Vol.24 (9), p.1359-1364
Main Authors: Chang, Mun Seog, Choi, Moon Jung, Park, Soo Yeon, Park, Seong Kyu
Format: Article
Language:English
Subjects:
Animals
B16F1 cell
Biological and medical sciences
Biological Products - pharmacology
Biological Products - therapeutic use
Cell Line, Tumor
Cell Survival - drug effects
Gene Expression Regulation - drug effects
General pharmacology
Hoelen
Medical sciences
Melanins - biosynthesis
melanogenesis
Melanoma - drug therapy
Melanoma - metabolism
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Mice
Monophenol Monooxygenase - metabolism
Oxidoreductases - genetics
Oxidoreductases - metabolism
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Poria
tyrosinase related protein
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Summary:Melanin synthesis is regulated by melanogenic proteins, such as tyrosinase, tyrosinase-related protein 1 (TRP-1) and TRP-2. The effects of Hoelen extract on melanogenesis were investigated in B16Fl murine melanoma cells. Specifically, tyrosinase activity, cell viability and melanin content were assayed, and western blotting and RT-PCR for tyrosinase, TRP-1 and TRP-2 conducted. The results show that Hoelen significantly inhibited melanin synthesis through inhibition of TRP-2 expression, while it did not affect tyrosinase activity or its expression. Taken together, RT-PCR results showed that the depigmentation effect of Hoelen may be due to inhibition of TRP-2 gene transcription. These results suggest that Hoelen may be a useful inhibitor for the attenuation of melanogenesis and hyperpigmentation in skin cells. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.3123