Amelioration of oxidative stress by dandelion extract through CYP2E1 suppression against acute liver injury induced by carbon tetrachloride in sprague-dawley rats

The protective effects of common dandelion leaf water extract (DLWE) were investigated by carbon tetrachloride (CCl₄) induced hepatitis in Sprague-Dawley rats. The animals were divided into five groups: normal control, DLWE control, CCl₄ control, and two DLWE groups (0.5 and 2 g/kg bw). After 1 week...

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Bibliographic Details
Published in:Phytotherapy research 2010-09, Vol.24 (9), p.1347-1353
Main Authors: Park, Chung Mu, Cha, Yeon Suk, Youn, Hyun Joo, Cho, Chung Won, Song, Young Sun
Format: Article
Language:English
Subjects:
Animals
antiinflammatory
Antioxidants - metabolism
Antioxidants - pharmacology
Antioxidants - therapeutic use
Biological and medical sciences
Carbon Tetrachloride
Chemical and Drug Induced Liver Injury - drug therapy
Chemical and Drug Induced Liver Injury - metabolism
Cholesterol - blood
CYP2E1
Cytochrome P-450 CYP2E1 Inhibitors
dandelion leaf water extract
Enzymes - metabolism
fas Receptor - genetics
fas Receptor - metabolism
Gene Expression Regulation - drug effects
General pharmacology
Inflammation - drug therapy
Inflammation - etiology
Lipid Peroxidation - drug effects
Liver - drug effects
Liver - metabolism
Male
Medical sciences
oxidative stress
Oxidative Stress - drug effects
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phytotherapy
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Plant Leaves
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Taraxacum
Taraxacum officinale
Triglycerides - blood
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
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Summary:The protective effects of common dandelion leaf water extract (DLWE) were investigated by carbon tetrachloride (CCl₄) induced hepatitis in Sprague-Dawley rats. The animals were divided into five groups: normal control, DLWE control, CCl₄ control, and two DLWE groups (0.5 and 2 g/kg bw). After 1 week of administering corresponding vehicle or DLWE, a single dose of CCl₄ (50% CCl₄/olive oil; 0.5 mL/kg bw) was administered 24 h before killing in order to produce acute liver injury. The DLWE treatment significantly decreased CCl₄-induced hepatic enzyme activities (AST, ALT and LDH) in a dose dependent manner. Also, the obstructed release of TG and cholesterol into the serum was repaired by DLWE administration. Hepatic lipid peroxidation was elevated while the GSH content and antioxidative enzyme activities were reduced in the liver as a result of CCl₄ administration, which were counteracted by DLWE administration. Furthermore, the hepatocytotoxic effects of CCl₄ were confirmed by significantly elevated Fas and TNF-? mRNA expression levels, but DLWE down-regulated these expressions to the levels of the normal control. Highly up-regulated cytochrome P450 2E1 was also lowered significantly in the DLWE groups. These results indicate that DLWE has a protective effect against CCl₄-induced hepatic damage with at least part of its effect being attributable to the attenuation of oxidative stress and inflammatory processes resulting from cytochrome P450 activation by CCl₄. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.3121