Identification of Each Human Chromosome with a Modified Giemsa Stain

Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the pattern is quite similar to that obtained with qu...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1971-08, Vol.173 (3999), p.821-822
Main Authors: Patil, Shivanand R., Merrick, Suzanne, Lubs, Herbert A.
Format: Article
Language:English
Subjects:
Buffers
Centromeres
Chromosomes
Fluorescence
Fluorescent Dyes
Human chromosomes
Humans
Hydrogen-Ion Concentration
Karyotyping
Methods
Mustards
Quinacrine
Staining and Labeling
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Summary:Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the pattern is quite similar to that obtained with quinacrine mustard fluorescence staining. Certain regions, such as the paracentric constrictions in chromosomes A1 and C9, and the distal end of the long arm of the Y chromosome stain differently with the Giemsa 9 technique. The technique is considerably simpler than the quinacrine mustard fluorescence technique and identification of homologs is also easier than in cells stained by the latter.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.173.3999.821