Development of Oxidase Activity by Human Bone Marrow Granulocytes

To study the stage of development of oxidase activity responsible for the respiratory burst in human granulocytes, we investigated the effect of phorbol myris-tate acetate (PMA) and fluoride (F ) on nitroblue tetrazolium (NBT) reduction by bone marrow and circulating granulocytic cells in 13 subject...

Full description

Saved in:
Bibliographic Details
Published in:Blood 1979-08, Vol.54 (2), p.429-439
Main Authors: Zakhireh, Behnam, Root, Richard K.
Format: Article
Language:English
Subjects:
Bone Marrow - enzymology
Cell Differentiation
Cytochalasin B - pharmacology
Fluorides - pharmacology
Granulocytes - enzymology
Granulocytes - ultrastructure
Hematopoiesis
Humans
Nitroblue Tetrazolium - metabolism
Oxidation-Reduction
Oxidoreductases - biosynthesis
Vacuoles - ultrastructure
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To study the stage of development of oxidase activity responsible for the respiratory burst in human granulocytes, we investigated the effect of phorbol myris-tate acetate (PMA) and fluoride (F ) on nitroblue tetrazolium (NBT) reduction by bone marrow and circulating granulocytic cells in 13 subjects without leukocyte functional disorders and a patient with chronic granulomatous disease (CGD). Myeloblasts, promyelocytes, and myelocytes did not reduce NBT. Metamyelocytes showed minimal activity that improved with further maturation of the cell. Morphologically mature bone marrow polymorphonuclear leukocytes (PMNs) had significantly less NBT-reducing capacity than peripheral blood cells. Inhibition of phagocytosis by cytochalasin B completely abolished dye reduction under resting conditions. PMA-stimulated NBT reduction, however, was not affected. Thus, ingestion of NBT was not a prerequisite for its reduction. None of the PMNs from the CGD patient reduced NBT. These results indicate that either oxidase activity or the ability of cells to respond to activating stimuli does not develop until the metamyelocyte stage, and furthermore, a disparity between the morphological and functional maturation exists in bone marrow granulocytes.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V54.2.429.429