A Comparison of RNA with DNA in Template-Directed Synthesis

Nonenzymatic template-directed copying of RNA sequences rich in cytidylic acid using nucleoside 5'-(2-methylimidazol-1-yl phosphates) as substrates is substantially more efficient than the copying of corresponding DNA sequences. However, many sequences cannot be copied, and the prospect of repl...

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Bibliographic Details
Published in:Helvetica chimica acta 2000-08, Vol.83 (8), p.1678-1684
Main Authors: Zielinski, Magdalene, Kozlov, Igor A., Orgel, Leslie E.
Format: Article
Language:English
Subjects:
Base Pairing
Base Sequence
Cytidine Monophosphate - chemistry
Deoxyribonucleotides - chemistry
Directed Molecular Evolution
DNA - chemistry
DNA Primers
Evolution, Molecular
Exobiology
Oligonucleotides - chemical synthesis
Ribonucleotides - chemistry
RNA - chemistry
Space life sciences
Templates, Genetic
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Summary:Nonenzymatic template-directed copying of RNA sequences rich in cytidylic acid using nucleoside 5'-(2-methylimidazol-1-yl phosphates) as substrates is substantially more efficient than the copying of corresponding DNA sequences. However, many sequences cannot be copied, and the prospect of replication in this system is remote, even for RNA. Surprisingly, wobble-pairing leads to much more efficient incorporation of G opposite U on RNA templates than of G opposite T on DNA templates.
ISSN:0018-019X
1522-2675
DOI:10.1002/1522-2675(20000809)83:8<1678::AID-HLCA1678>3.0.CO;2-P