Development, Characterization and Cross Species Amplification of Polymorphic Microsatellite Markers from Expressed Sequence Tags of Turmeric (Curcuma longa L.)

Expressed sequence tags (ESTs) from turmeric ( Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The av...

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Bibliographic Details
Published in:Molecular biotechnology 2010-02, Vol.44 (2), p.140-147
Main Authors: Siju, S., Dhanya, K., Syamkumar, S., Sasikumar, B., Sheeja, T. E., Bhat, A. I., Parthasarathy, V. A.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Biological Techniques
Biotechnology
Cell Biology
Chemistry
Chemistry and Materials Science
Curcuma - genetics
Curcuma longa
DNA, Plant - genetics
Electrophoresis
Expressed Sequence Tags
Flowers & plants
Fundamental and applied biological sciences. Psychology
Human Genetics
Microsatellite Repeats - genetics
Polymerase Chain Reaction
Polymorphism
Protein Science
Satellite DNA
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Summary:Expressed sequence tags (ESTs) from turmeric ( Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST–SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-009-9222-4