Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: Evaluation of a novel biocatalyst in organic media

The encapsulation of crosslinked enzyme aggregates (CLEA) of penicillin G acylase into a very rigid polymeric matrix based on polyvinyl alcohol (LentiKats) has been used successfully to improve the inadequate mechanical properties of CLEA. This encapsulation decreased CLEA activity by only around 40...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2004-06, Vol.86 (5), p.558-562
Main Authors: Wilson, Lorena, Illanes, Andrés, Pessela, Benevides C. C., Abian, Olga, Fernández-Lafuente, Roberto, Guisán, José M.
Format: Article
Language:English
Subjects:
Absorption
Antibiotics
biocatalysts
Biological and medical sciences
Biotechnology
Catalysis
Cephalosporins - chemical synthesis
Cross-Linking Reagents - chemistry
crosslinked enzyme aggregates
Dimerization
Encapsulation
entrapment
Enzyme Activation
Enzyme Stability
enzymes in organic solvents
Enzymes, Immobilized - chemistry
Fundamental and applied biological sciences. Psychology
hydrogel
Hydrogels - chemistry
Hydrolysis
immobilization
Immobilized enzymes
LentiKats
Organic Chemicals - chemistry
penicillin acylase
Penicillin Amidase - chemistry
penicillin G acylase
phenylacetic acid
Polyvinyl alcohol
Polyvinyl Alcohol - chemistry
Q1
Solvents
Temperature
Thermal stability
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Summary:The encapsulation of crosslinked enzyme aggregates (CLEA) of penicillin G acylase into a very rigid polymeric matrix based on polyvinyl alcohol (LentiKats) has been used successfully to improve the inadequate mechanical properties of CLEA. This encapsulation decreased CLEA activity by only around 40%. As compensation, a significant improvement in the stability of the CLEA in the presence of organic solvents was detected. This could be related to the highly hydrophilic environment inside the LentiKats biocatalysts: Partition experiments showed that the concentration of dioxane inside LentiKats was lower than in the reaction medium. In fact, thermal stability was about the same as in the corresponding CLEA. This permitted great improvement in the reaction rate for thermodynamically controlled synthesis of a model antibiotic (using phenylacetic acid and 7‐amino‐deacetoxycefalosporanic acid). Even more importantly, yields could be improved by using LentiKats‐encapsulated CLEA, very likely by a favorable product/substrate partition. Thus, this very simple technique not only provides an efficient technique for solving the mechanical stability problem associated with CLEA, but also greatly improves the behavior of CLEA in organic media. © 2004 Wiley Periodicals, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.20107