Near-optimal Conditions for the In Vitro Culture of Hemopoietic Progenitor Cells in Bone Marrow from the Rat

In vitro techniques for the culture of hemopoietic stem cells and committed hemopoietic progenitor cells in rat bone marrow have not been adequately described in the literature. In the present investigations, and using commercially available hemopoietic cytokines and growth factors, the conditions r...

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Bibliographic Details
Published in:Toxicologic pathology 2009-02, Vol.37 (2), p.170-174
Main Authors: MOLYNEUX, Gemma, RIZZO, Sian, TURTON, John, PHUL, Parvinder, GIBSON, Frances
Format: Article
Language:English
Subjects:
Animals
Animals, Outbred Strains
Biological and medical sciences
Bone Marrow Cells - cytology
Cell Culture Techniques - methods
Cells, Cultured
Colony-Forming Units Assay - methods
Culture Media - chemistry
Female
Femur - cytology
Hematopoietic Stem Cells - cytology
Medical sciences
Rats
Rats, Inbred F344
Rats, Wistar
Recombinant Proteins - metabolism
Stem Cell Factor - genetics
Stem Cell Factor - metabolism
Time Factors
Toxicology
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Summary:In vitro techniques for the culture of hemopoietic stem cells and committed hemopoietic progenitor cells in rat bone marrow have not been adequately described in the literature. In the present investigations, and using commercially available hemopoietic cytokines and growth factors, the conditions required to perform long-term bone marrow culture (LTBMC) using rat femoral bone marrow were studied, in conjunction with the colony-forming unit cell assay (CFU-C), to quantify the number of progenitor cells. CFU-C production by LTBMCs, set up using Iscove's modified Dulbecco's medium supplemented with fetal calf serum and horse serum, ceased after week 2 of culture. However, the duration of CFU-C production was significantly increased by supplementing LTBMCs with the cytokine recombinant mouse stem cell factor or recombinant rat stem cell factor.
ISSN:0192-6233
1533-1601
DOI:10.1177/0192623308328133