ERIC-PCR identification of the spread of airborne Escherichia coli in pig houses

To understand the spread of microbial aerosols in pig houses, with Escherichia coli ( E. coli) as indicator, the airborne E. coli in 4 pig houses and their surroundings at different points 10, 50 m upwind and 10, 50, 100, 200 and 400 m downwind respectively from the pig houses were collected, and th...

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Bibliographic Details
Published in:The Science of the total environment 2010-02, Vol.408 (6), p.1446-1450
Main Authors: Yuan, W., Chai, T.J., Miao, Z.M.
Format: Article
Language:English
Subjects:
Aerosols
Air Microbiology
Air Pollutants - analysis
Air Pollutants - isolation & purification
Air Pollution, Indoor - analysis
Airborne Escherichia coli
Amplification
Animal Husbandry
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
DNA, Bacterial - isolation & purification
DNA, Intergenic - isolation & purification
Ecotoxicology, biological effects of pollution
Environmental Monitoring - methods
ERIC-PCR
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli - isolation & purification
Feces - microbiology
Fundamental and applied biological sciences. Psychology
General aspects
Houses
Housing, Animal
Indoor
Microbial aerosols
Microorganisms
Pig houses
Polymerase Chain Reaction - methods
Similarity
Spreads
Swine
Terrestrial environment, soil, air
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Summary:To understand the spread of microbial aerosols in pig houses, with Escherichia coli ( E. coli) as indicator, the airborne E. coli in 4 pig houses and their surroundings at different points 10, 50 m upwind and 10, 50, 100, 200 and 400 m downwind respectively from the pig houses were collected, and the concentrations were calculated at each sampling point. Furthermore, the feces of pigs were collected to separate E. coli. The ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction) technology was used to amplify the isolated E. coli DNA samples, then the amplified results were analyzed by NTSYS-pc (Version 2.10) to identify the similarity of isolated E. coli. The results showed that the airborne E. coli concentrations in indoor air of the 4 pig houses (21–35 CFU m − 3 air) were much higher than those in upwind and downwind air ( P < 0.05), but there were no significant differences ( P > 0.05) at downwind distances. The ERIC-PCR results also showed that 52.4% of the fecal E. coli (four houses being respectively 2/4, 50%; 2/4, 50%; 3/6, 50%; 4/7, 57.1%) were identical to the indoor airborne E. coli isolates, and there was more than 90% similarity between the majority of E. coli (50%, 21/42) isolated from downwind air at 10, 50, 100 and 200 m and those from indoor air or feces. It could be concluded that the aerosols in pig houses can spread to the surroundings, and thus effective measures should be taken to control and minimize the spread of microbial aerosols.
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2009.12.019