Synthesis of cyclopropane fatty acids in Lactobacillus helveticus and Lactobacillus sanfranciscensis and their cellular fatty acids changes following short term acid and cold stresses

An implemented GC method to separate and quantify the cell cyclopropane fatty acids lactobacillic (C19cyc11) and dehydrosterculic (C19cyc9) was used to study the adaptive response to sublethal acid and cold stresses in Lactobacillus helveticus and Lactobacillus sanfranciscensis. The comparison of th...

Full description

Saved in:
Bibliographic Details
Published in:Food microbiology 2010-06, Vol.27 (4), p.493-502
Main Authors: Montanari, Chiara, Sado Kamdem, Sylvain L., Serrazanetti, Diana I., Etoa, François-Xavier, Guerzoni, M. Elisabetta
Format: Article
Language:English
Subjects:
Acid stress
acid treatment
Adaptation, Physiological
aerobic conditions
anaerobic conditions
Biological and medical sciences
biosynthesis
Chromatography, Gas
Cold stress
Cold Temperature
culture media
Cyclopropane fatty acids
Cyclopropanes - analysis
cyclopropenoid fatty acids
cytoplasm
Fatty Acids - analysis
Fatty Acids - biosynthesis
Food industries
Food Microbiology
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
lactic acid bacteria
Lactobacillus - metabolism
Lactobacillus - physiology
Lactobacillus helveticus
Lactobacillus helveticus - metabolism
Lactobacillus helveticus - physiology
Lactobacillus sanfranciscensis
Membrane Lipids - analysis
Microbial Viability
Oxidative Stress
Oxygen - metabolism
secondary metabolites
short chain fatty acids
starter cultures
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An implemented GC method to separate and quantify the cell cyclopropane fatty acids lactobacillic (C19cyc11) and dehydrosterculic (C19cyc9) was used to study the adaptive response to sublethal acid and cold stresses in Lactobacillus helveticus and Lactobacillus sanfranciscensis. The comparison of the composition of cellular fatty acids of the two strains and their changes after 2 h of stress exposure under micro-aerobic and anaerobic conditions indicated that the aerobic biosynthetic pathway for unsaturated fatty acids is prevalent in L. sanfranciscensis, while the anaerobic pathway is prevalent in L. helveticus. Indeed in the latter strain, in the presence of a source of oleic acid and under micro-aerobic conditions, C18:1n11 and its post-synthetic derivative C19cyc11 accounted for overall proportion ranging from 52 to 28% of the total FAs. On the other hand L. sanfranciscensis synthesizes by aerobic pathway C18:1n9 and transforms it to C19cyc9. However in this species the cumulative level of these two FAs did not exceed 30%. The relevant proportion of dodecanoic acid in the latter species suggests that carbon chain shortening is the principal strategy of L. sanfranciscensis to modulate fluidity or chemico-physical properties of the membranes.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2009.12.003