Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens

A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the...

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Bibliographic Details
Published in:International immunopharmacology 2010-03, Vol.10 (3), p.364-370
Main Authors: Na, Ye Seul, Kim, Woo Jung, Kim, Sung Min, Park, Jae Kweon, Lee, Sae Mi, Kim, Sung Oog, Synytsya, Andriy, Park, Yong Il
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - chemistry
Adjuvants, Immunologic - isolation & purification
Adjuvants, Immunologic - pharmacology
Animals
Biological and medical sciences
Capsosiphon fulvescens
Chlorophyta - chemistry
Coloring Agents
Cyclooxygenase 2 - metabolism
Cytokines - biosynthesis
Dose-Response Relationship, Drug
Immunostimulation
Interleukin-6 - biosynthesis
Korea
Macrophages
Macrophages - drug effects
Macrophages - immunology
Medical sciences
Mice
Molecular Weight
Monosaccharides - analysis
Nitric Oxide Synthase Type II - metabolism
Pharmacology. Drug treatments
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
Sulfated polysaccharide
Tetrazolium Salts
Thiazoles
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean Capsosiphon fulvescens by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The purified SPS-CF was shown to be a glucuronogalactomannan with a molecular mass of 385 kDa and the monosaccharide composition of the SPS-CF was determined to be mannose (55.4% in mole percentage), galactose (25.3%), glucuronic acid (16.3%), and arabinose (0.8%). Fourier-transform infrared and elemental analysis indicated that the purified SPS-CF is a sulfated polysaccharide containing significant amount of sulfate esters (5.7% in mass). Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 µg/ml in RAW264.7 murine macrophages. These results suggest that the sulfated SPS-CF isolated from C. fulvescens has potent immunostimulating activity.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2009.12.011