Expression of metalloproteinases and their tissue inhibitors in gingiva affected by hereditary gingival fibromatosis: analysis of three cases within a family

Background and Objective:  Hereditary gingival fibromatosis (HGF) is a benign disorder manifested by fibrous enlargement of keratinized gingiva. Evidence exists concerning the role of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in mediating normal and pathological processes,...

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Bibliographic Details
Published in:Journal of periodontal research 2009-12, Vol.44 (6), p.714-717
Main Authors: Da Ros Gonçalves, L., Oliveira, G. A. P., Borojevic, R., Otazu, I. B., Feres-Filho, E. J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biopsy
Dentistry
Enzyme Precursors - analysis
Enzyme Precursors - genetics
Fibromatosis, Gingival - enzymology
Fibromatosis, Gingival - genetics
Gene Expression Regulation, Enzymologic - genetics
gingiva
Gingiva - enzymology
Gingiva - pathology
gingival fibromatosis
Humans
matrix metalloproteinase
Matrix Metalloproteinase 1 - analysis
Matrix Metalloproteinase 1 - genetics
Matrix Metalloproteinase 2 - analysis
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 9 - analysis
Matrix Metalloproteinase 9 - genetics
Matrix Metalloproteinases - analysis
Matrix Metalloproteinases - genetics
Medical sciences
Otorhinolaryngology. Stomatology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
tissue inhibitor of metalloproteinase
Tissue Inhibitor of Metalloproteinase-1 - analysis
Tissue Inhibitor of Metalloproteinase-1 - genetics
Tissue Inhibitor of Metalloproteinase-2 - analysis
Tissue Inhibitor of Metalloproteinase-2 - genetics
Tissue Inhibitor of Metalloproteinases - analysis
Tissue Inhibitor of Metalloproteinases - genetics
Transcription, Genetic - genetics
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Summary:Background and Objective:  Hereditary gingival fibromatosis (HGF) is a benign disorder manifested by fibrous enlargement of keratinized gingiva. Evidence exists concerning the role of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in mediating normal and pathological processes, including HGF. Nevertheless, there are few and contradictory results on the analysis of MMPs and TIMPs transcripts in this pathology. Material and Methods:  We studied the expression of the transcripts encoding MMP‐1, ‐2 and ‐9 and TIMP‐1 and ‐2 in gingival biopsies, obtained from three cases of HGF within a family, by semi‐quantitative reverse transcriptase‐polymerase chain reaction analysis. Samples were also processed for gelatin zymography. Results:  Except for MMP‐9, most transcripts presented a higher level of expression in biopsies from HGF patients compared with control subjects. Accordingly, MMP‐9 gelatinase activity was detected at low and similar levels among samples. Moreover, MMP‐2 enzymatic activity was not detected at all. Conclusion:  The mRNA expression of MMP‐1 and ‐2 and TIMP‐1 and ‐2 does not explain the gingival overgrowth presented in these cases. In addition, it is suggested that the gene expression of those molecules in the course of HGF is regulated at the translational or post‐translational level.
ISSN:0022-3484
1600-0765
DOI:10.1111/j.1600-0765.2008.01180.x