Development of a blocking ELISA using a recombinant glycoprotein for the detection of antibodies to vesicular stomatitis New Jersey virus

A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value...

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Bibliographic Details
Published in:Journal of virological methods 2010-03, Vol.164 (1), p.96-100
Main Authors: Heo, Eun-Jeong, Lee, Hyang-Sim, Jeoung, Hye-Young, Ko, Hyo-Rim, Kweon, Chang-Hee, Ko, Young-Joon
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Viral - blood
Antigens, Viral - genetics
Baculoviridae - genetics
Baculovirus
Biological and medical sciences
Blocking ELISA
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - virology
Cell Line
Clinical Laboratory Techniques - methods
Enzyme-Linked Immunosorbent Assay - methods
Fundamental and applied biological sciences. Psychology
Glycoproteins - genetics
Horse Diseases - diagnosis
Horse Diseases - virology
Horses
Microbiology
Recombinant glycoprotein
Recombinant Proteins - genetics
Rhabdoviridae Infections - diagnosis
Rhabdoviridae Infections - veterinary
Rhabdoviridae Infections - virology
Sensitivity and Specificity
Spodoptera
Techniques used in virology
Vesicular stomatitis New Jersey virus
Vesicular stomatitis New Jersey virus - immunology
Virology
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Summary:A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value of 73% inhibition, the R-GP ELISA exhibited 99.1% specificity for naive sera from cattle and horses. It did not cross-react with VSV-Indiana (VSV-IN) positive sera and differentiated from foot-and-mouth disease and swine vesicular disease. Taken together, this is the first report that the R-GP has a potential to be used as a diagnostic antigen in place of the native GP for the detection of antibodies to VSV-NJ in cattle and horses.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2009.12.005