Oxidative Stress Biomarkers in the Digestive Gland of Theba pisana Exposed to Heavy Metals

The in vivo toxic effects of sublethal treatment of 40 and 80% of 48-h LD₅₀ of topically applied trace metals [copper (Cu), lead (Pb), and zinc (Zn)] on oxidative stress biomarkers in the digestive gland of Theba pisana were examined. Oxidative individual perturbations were assessed by measuring non...

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Bibliographic Details
Published in:Archives of environmental contamination and toxicology 2010-04, Vol.58 (3), p.828-835
Main Authors: Radwan, M. A, El-Gendy, K. S, Gad, A. F
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Animals
Antioxidants
Applied ecology
Biocompatibility
Biological and medical sciences
Biomarkers
Breath tests
Catalase - metabolism
Chemicals
Contamination
Copper
Copper - toxicity
Digestive system
Digestive System - drug effects
Digestive System - metabolism
Earth and Environmental Science
Ecosystem
Ecosystems
Ecotoxicology
Ecotoxicology, biological effects of pollution
Environment
Environmental Chemistry
Environmental Health
Environmental Monitoring
Enzymes
Exposure
Fundamental and applied biological sciences. Psychology
General aspects
Glutathione - metabolism
Glutathione Peroxidase - metabolism
Glutathione Transferase - metabolism
Heavy metals
Humidity
Lead (metal)
Lead - toxicity
Lethal Dose 50
Lipid peroxidation
Metals
Metals, Heavy - toxicity
Mollusks
Monitoring/Environmental Analysis
Oxidation
Oxidative Stress
Pollutants
Pollution
Snails
Snails - drug effects
Snails - metabolism
Soil Science & Conservation
Stresses
Studies
Techniques
Toxicity
Toxicology
Zinc
Zinc - toxicity
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Summary:The in vivo toxic effects of sublethal treatment of 40 and 80% of 48-h LD₅₀ of topically applied trace metals [copper (Cu), lead (Pb), and zinc (Zn)] on oxidative stress biomarkers in the digestive gland of Theba pisana were examined. Oxidative individual perturbations were assessed by measuring nonenzymatic (glutathione; GSH) and enzymatic (catalase, CAT; glutathione peroxidase, GPx; and glutathione-S-transferase, GST) antioxidants in digestive gland of the snails. Lipid peroxidation (LPO) was also evaluated as a marker of cell damage. The results indicated that the copper ion was the most potent metal against this snail, followed by zinc and lead, for which the corresponding LD₅₀ values were 37.88, 261.72, and 652.55 μg/snail, respectively. The no-observed effect concentration (NOEC) values for Cu, Zn, and Pb were 10, 50, and 500 μg/snail, respectively, and the corresponding lowest-observed effect concentration (LOEC) values were 50, 100, and 1000 μg/snail. All trace metals resulted in a significant increase in the level of LPO, whereas a significant decline in the content of GSH was observed when compared with untreated controls. Treatment with both sublethal doses of the metals caused significant increase in CAT activity, except in the case of 40% LD₅₀ Zn and 80% LD₅₀ Cu, which exhibited no alteration in CAT when compared to control animals. GPx was significantly increased in snails exposed to 40% LD₅₀ Cu and Pb as well as 80% LD₅₀ Cu. However, an opposite effect was observed in snails exposed to 80% LD₅₀ Pb and in either 40 or 80% LD₅₀ of Zn-intoxicated animals. Treatment with Pb at two sublethal doses significantly increased GST activity, whereas treatment the animal with Cu caused significant inhibition in this enzyme. Snails exposed to 40% LD₅₀ Zn showed significant enhancement of GST, whereas snails exposed to 80% LD₅₀ showed ignificantly reduced GST activity. Biphasic responses were observed for CAT, GPx, and GST activities in snails exposed to Cu, Pb, and Zn, respectively. This study suggests that upregulation of the antioxidant enzyme activities, elevation of LPO, and the reduction in GSH content is related to oxidative stress in this species that could be useful as biomarkers for the evaluation of contaminated terrestrial ecosystems.
ISSN:0090-4341
1432-0703
DOI:10.1007/s00244-009-9380-1