Trypanocidal, leishmanicidal and antifungal potential from marine red alga Bostrychia tenella J. Agardh (Rhodomelaceae, Ceramiales)

Specimens of the red alga Bostrychia tenella J. Agardh (Rhodomelaceae, Ceramiales) were collected from the São Paulo coast and submitted to room temperature solvent extraction. The resulting extract was fractionated by partitioning with organic solvent. The n-hexane (BT-H) and dichloromethane (BT-D)...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2010-09, Vol.52 (5), p.763-769
Main Authors: de Felício, Rafael, de Albuquerque, Sérgio, Young, Maria Cláudia Marx, Yokoya, Nair Sumie, Debonsi, Hosana Maria
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Antifungal Agents - isolation & purification
Antifungal Agents - pharmacology
Antifungal potential
Biological and medical sciences
Bostrychia tenella
Ceramiales
Cladosporium cladosporioides
Cladosporium sphaerospermum
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
GC/MS
General pharmacology
Leishmania - drug effects
Leishmania amazonensis
Marine red algae
Medical sciences
Pharmacology. Drug treatments
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Rhodomelaceae
Rhodophyta - chemistry
Trypanocidal Agents - isolation & purification
Trypanocidal Agents - pharmacology
Trypanocidal and leishmanicidal activity
Trypanosoma cruzi
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Summary:Specimens of the red alga Bostrychia tenella J. Agardh (Rhodomelaceae, Ceramiales) were collected from the São Paulo coast and submitted to room temperature solvent extraction. The resulting extract was fractionated by partitioning with organic solvent. The n-hexane (BT-H) and dichloromethane (BT-D) fractions showed antiprotozoal potential in biological tests with Trypanosoma cruzi and Leishmania amazonensis and presented high activity in an antifungal assay with the phytopathogenic fungi Cladosporium cladosporioides and Cladosporium sphaerospermum. Chromatography methods were used to generate subfractions from BT-H (H01 to H11) and from BT-D (D01 to D19). The subfractions were analyzed by gas chromatography–mass spectrometry (GC/MS), and the substances were identified by retention index (Kovats) and by comparison to databases of commercial mass spectra. The volatile compounds found in marine algae were identified as fatty acids, low molecular mass hydrocarbons, esters and steroids; some of these have been previously described in the literature based on other biological activities. Moreover, uncommon substances, such as neophytadiene were also identified. In a trypanocidal assay, fractions BT-H and BT-D showed IC 50 values of 16.8 and 19.1 μg/mL, respectively, and were more active than the gentian violet standard (31 μg/mL); subfractions H02, H03, D01 and D02 were active against L. amasonensis, exhibiting IC 50 values of 1.5, 2.7, 4.4, and 4.3 μg/mL, respectively (standard amphotericin B: IC 50 = 13 μg/mL). All fractions showed antifungal potential. This work reports the biological activity and identification of compounds by GC/MS for the marine red alga B. tenella for the first time.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2010.02.018