Medium optimization for hen egg white lysozyme production by recombinant Aspergillus niger using statistical methods

Statistics‐based experimental design was used to investigate the effect of medium components (starch, peptone, ammonium sulfate, yeast extract, and CaCl2 · 2H2O) on hen's egg white lysozyme production by Aspergillus niger HEWL WT‐13‐16. A 25−1 fractional factorial design augmented with center p...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2005-06, Vol.90 (6), p.754-760
Main Authors: Gheshlaghi, R., Scharer, J.M., Moo-Young, M., Douglas, P.L.
Format: Article
Language:English
Subjects:
Albumen
Algorithms
Ammonium sulfate
Animals
Aspergillus niger
Aspergillus niger - genetics
Aspergillus niger - growth & development
Aspergillus niger - metabolism
Biological and medical sciences
Bioreactors - microbiology
Biotechnology
Cell Culture Techniques - methods
Chickens
Computer Simulation
Culture Media - metabolism
Eggs
experimental designs
Fundamental and applied biological sciences. Psychology
hen egg white lysozyme
Lysozyme
Media (culture)
medium optimization
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Models, Biological
Muramidase - biosynthesis
Muramidase - genetics
peptone
Protein Engineering - methods
Q1
Q2
Recombinant Proteins - biosynthesis
Research methodology
response surface methodology
Starch
Statistical methods
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Summary:Statistics‐based experimental design was used to investigate the effect of medium components (starch, peptone, ammonium sulfate, yeast extract, and CaCl2 · 2H2O) on hen's egg white lysozyme production by Aspergillus niger HEWL WT‐13‐16. A 25−1 fractional factorial design augmented with center points revealed that peptone, starch, and ammonium sulfate were the most significant factors, whereas the other factors were not important within the levels tested. The method of steepest ascent was used to approach the proximity of optimum. This task was followed by a central composite design to develop a response surface for medium optimization. The optimum medium composition for lysozyme production was found to be: starch 34 g L−1, peptone 34 g L−1, ammonium sulfate 11.9 g L−1, yeast extract 0.5 g L−1, and CaCl2 · 2H2O 0.5 g L−1. This medium was projected to produce, theoretically, 212 mg L−1 lysozyme. Using this medium, an experimental maximum lysozyme concentration of 209 ± 18 mg L−1 verified the applied methodology. © 2005 Wiley Periodicals, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.20474