Characterization of populations of entomopathogenic nematodes isolated at diverse sites across Israel

Entomopathogenic nematodes (EPNs) are known as effective biological agents against soil-dwelling stages of insect pests. In the present study new EPN populations from the Steinernematidae and Heterorhabditidae families were isolated at different locations in Israel. The newly isolated populations we...

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Bibliographic Details
Published in:Phytoparasitica 2010-02, Vol.38 (1), p.39-52
Main Authors: Salame, L, Glazer, I, Miqaia, N, Chkhubianishvili, T
Format: Article
Language:English
Subjects:
Agriculture
Animal populations
Animal reproduction
Bioassays
biological control
biological control agents
Biomedical and Life Sciences
dehydration (animal physiology)
dose response
Ecology
entomopathogenic nematodes
Galleria mellonella
geographical distribution
heat stress
Heterorhabditidae
Heterorhabditis
Insect control
insect pests
lethal dose
Life Sciences
motility
Nematoda
Nematodes
pathogenicity
Plant Pathology
plant pests
Plant Sciences
selection criteria
Steinernema feltiae
Steinernematidae
strain differences
water stress
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Summary:Entomopathogenic nematodes (EPNs) are known as effective biological agents against soil-dwelling stages of insect pests. In the present study new EPN populations from the Steinernematidae and Heterorhabditidae families were isolated at different locations in Israel. The newly isolated populations were subjected to a series of bioassays for beneficial traits, for selection of a superior isolate for biological control of insect pests. Nematode motility was measured in sand columns, with and without the susceptible insect Galleria mellonella . Infectivity was evaluated using an invasion rate assay, as well as a dose response assay in which LD 50 values were calculated. Nematode tolerance to environmental stresses was examined in heat and desiccation tolerance assays. The reproductive potentials of the new isolates were assessed in cadavers of G. mellonella . All Steinernematid populations were identified as Steinernema feltiae and the Heterorhabditids as Heterorhabditis sp. Large variation between the various populations was recorded in all assays. In order to identify a superior isolate, each strain was scored as 1 if its performance did not differ significantly from the highest level recorded in the particular assay; −1 if its performance did not differ significantly from the lowest level; and 0 if its performance was between the highest and lowest levels or did not differ from either. The scores for the different traits were then summed for each population and the totals were compared. The best score (+4) was obtained for a S. feltiae population isolated in northern Israel. These results will be used for the utilization of the new population as a biological control agent.
ISSN:0334-2123
1876-7184
DOI:10.1007/s12600-009-0072-1