Characterization of a para-nitrophenol catabolic cluster in Pseudomonas sp. strain NyZ402 and construction of an engineered strain capable of simultaneously mineralizing both para- and ortho-nitrophenols

Pseudomonas sp. strain NyZ402 was isolated for its ability to grow on para-nitrophenol (PNP) as a sole source of carbon, nitrogen, and energy, and was shown to degrade PNP via an oxidization pathway. This strain was also capable of growing on hydroquinone or catechol. A 15, 818 bp DNA fragment exten...

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Bibliographic Details
Published in:Biodegradation (Dordrecht) 2010-07, Vol.21 (4), p.575-584
Main Authors: Wei, Qing, Liu, Hong, Zhang, Jun-Jie, Wang, Song-He, Xiao, Yi, Zhou, Ning-Yi
Format: Article
Language:English
Subjects:
Alcaligenes
Alcaligenes - enzymology
Analysis
Aquatic Pollution
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Benzoquinone
Biochemistry
Biodegradation
Biodegradation of pollutants
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Deoxyribonucleic acid
DNA
Engineered strain
Environment and pollution
Enzymes
Fundamental and applied biological sciences. Psychology
Gene Expression
Genetic Engineering
Genomics
Geochemistry
Industrial applications and implications. Economical aspects
Life Sciences
metabolism
Microbiology
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
Molecular Sequence Data
Neomycin
Nitrophenols - chemistry
Nitrophenols - metabolism
Original Paper
ortho-Nitrophenol
Oxidoreductases - genetics
Oxidoreductases - metabolism
para-Nitrophenol
Pseudomonas
Pseudomonas - genetics
Pseudomonas - metabolism
Quinone
Simultaneous degradation
Soil Science & Conservation
Terrestrial Pollution
Waste Management/Waste Technology
Waste Water Technology
Water Management
Water Pollution Control
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Summary:Pseudomonas sp. strain NyZ402 was isolated for its ability to grow on para-nitrophenol (PNP) as a sole source of carbon, nitrogen, and energy, and was shown to degrade PNP via an oxidization pathway. This strain was also capable of growing on hydroquinone or catechol. A 15, 818 bp DNA fragment extending from a 800-bp DNA fragment of hydroxyquinol 1,2-dioxygenase gene (pnpG) was obtained by genome walking. Sequence analysis indicated that the PNP catabolic gene cluster (pnpABCDEFG) in this fragment shared significant similarities with a recently reported gene cluster responsible for PNP degradation from Pseudomonas sp. strain WBC-3. PnpA is PNP 4-monooxygenase converting PNP to hydroquinone via benzoquinone in the presence of NADPH, and genetic analysis indicated that pnpA plays a key role in PNP degradation. pnpA1 present in the upstream of the cluster (absent in the cluster from strain WBC-3) encodes a protein sharing as high as 55% identity with PnpA, but was not involved in PNP degradation by either in vitro or in vivo analyses. Furthermore, an engineered strain capable of growing on PNP and ortho-nitrophenol (ONP) was constructed by introducing onpAB (encoding ONP monooxygenase and ortho-benzoquinone reductase which catalyzed the transformation of ONP to catechol) from Alcaligenes sp. strain NyZ215 into strain NyZ402.
ISSN:0923-9820
1572-9729
DOI:10.1007/s10532-009-9325-4