Extramedullary hematopoiesis is dysregulated in histamine-free histidine decarboxylase knockout (HDC⁻/⁻) mice

Objective and design In this study we investigated the role of histamine on the extramedullary hematopoiesis. Methods Male histidine decarboxylase knockout (HDC⁻/⁻) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 G...

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Bibliographic Details
Published in:Inflammation research 2010-06, Vol.59 (6), p.429-436
Main Authors: Horváth, Zsuzsanna, Pállinger, Éva, Horváth, Győző, Jelinek, Ivett, Veszely, Gizella, Fűrész, József, Falus, András, Buzás, Edit I
Format: Article
Language:English
Subjects:
Allergology
Animals
Biomedical and Life Sciences
Biomedicine
Cell Cycle - drug effects
Cells, Cultured
Colony-Forming Units Assay
Dermatology
Flow Cytometry
hematopoiesis
Hematopoiesis, Extramedullary - genetics
Hematopoiesis, Extramedullary - physiology
histamine
Histamine - physiology
Histidine Decarboxylase - genetics
Histidine Decarboxylase - physiology
IL-3 signaling
Immunology
Interleukin-3 - pharmacology
Male
Mice
Mice, Inbred BALB C
Mice, Knockout
Neurology
Original Research Paper
Pharmacology/Toxicology
Regeneration
Reverse Transcriptase Polymerase Chain Reaction
Rheumatology
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
spleen
Spleen - cytology
Spleen - drug effects
STAT5 Transcription Factor - biosynthesis
STAT5 Transcription Factor - genetics
Stem Cells - drug effects
Transgenic mice
Whole-Body Irradiation
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Summary:Objective and design In this study we investigated the role of histamine on the extramedullary hematopoiesis. Methods Male histidine decarboxylase knockout (HDC⁻/⁻) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student's t test, ANOVA, and Holm-Sidak post-hoc test were used. Results By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC⁻/⁻ mice (P < 0.01). The proliferative capacity and interleukin-3 signaling of stem cells were impaired in HDC⁻/⁻ mice. STAT5 mRNA expression was decreased in granulocyte-myeloid colonies by 72.9 ± 8.6% (P < 0.001), compared to the wild-type. Conclusions The absence of histamine adversely affects splenic hematopoiesis via direct and indirect mechanisms.
ISSN:1023-3830
1420-908X
DOI:10.1007/s00011-009-0114-7