Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst

Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2004-03, Vol.303 (5664), p.1669-1674
Main Authors: Hwang, Woo Suk, Ryu, Young June, Park, Jong Hyuk, Park, Eul Soon, Lee, Eu Gene, Koo, Ja Min, Jeon, Hyun Yong, Lee, Byeong Chun, Kang, Sung Keun, Kim, Sun Jong, Ahn, Curie, Hwang, Jung Hye, Park, Ky Young, Cibelli, Jose B., Moon, Shin Yong
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blastocyst
blastocysts
Cell lines
Cellular differentiation
Cloning
Donors
Embryonic stem cells
Embryos
General aspects
Genetic aspects
Individualized Instruction
Informed Consent
Medical sciences
Neurons
Oocytes
pluripotency
Pluripotent stem cells
Reproductive system
Somatic cell nuclear transfer
Somatic cells
Stem cell transplantation
Stem cells
Transplantation
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Summary:Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1094515