Translating Biomolecular Recognition into Nanomechanics

We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the can...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2000-04, Vol.288 (5464), p.316-318
Main Authors: Fritz, J., Baller, M. K., Lang, H. P., Rothuizen, H., Vettiger, P., Meyer, E., H.-J. Güntherodt, Ch. Gerber, Gimzewski, J. K.
Format: Article
Language:English
Subjects:
Adsorption
Animals
Antibody Specificity
Base Pair Mismatch
Base Pairing
Bending
Biological and medical sciences
Biotechnology
Cantilevers
Chemical Phenomena
Chemistry, Physical
Deoxyribonucleic acid
DNA
Feedback (Response)
Fundamental and applied biological sciences. Psychology
Goats
Gold - chemistry
Hydrogen Bonding
Immunoglobulin Constant Regions - chemistry
Ligands
Liquids
Methods. Procedures. Technologies
Molecular biology
Molecules
Nanotechnology
Nucleic Acid Hybridization
Nucleotides
Oligodeoxyribonucleotides - chemistry
Oligonucleotides
Others
Rabbits
Silicon
Silicon - chemistry
Staphylococcal Protein A - chemistry
Static Electricity
Stress, Mechanical
Thionucleotides - chemistry
Various methods and equipments
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Summary:We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.288.5464.316