The Birth of an Alternatively Spliced Exon: 3' Splice-Site Selection in Alu Exons

Alu repetitive elements can be inserted into mature messenger RNAs via a splicing-mediated process termed exonization. To understand the molecular basis and the regulation of the process of turning intronic Alus into new exons, we compiled and analyzed a data set of human exonized Alus. We revealed...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2003-05, Vol.300 (5623), p.1288-1291
Main Authors: Lev-Maor, Galit, Sorek, Rotem, Shomron, Noam, Ast, Gil
Format: Article
Language:English
Subjects:
Adenosine Deaminase - genetics
Alternative Splicing
Alu Elements - genetics
Biological and medical sciences
Cell Line
Cellular biology
Cloning, Molecular
Dinucleoside Phosphates - genetics
DNA, Antisense
Exons
Fundamental and applied biological sciences. Psychology
Genetic Disorders
Genetic mutation
Genetics
Genome, Human
Genomes
Glucosyltransferases - genetics
Humans
Introns
Messenger RNA
Mobile genetic elements
Molecular and cellular biology
Mutagenesis, Site-Directed
Nucleotides
Plasmids
Point Mutation
Polymerase Chain Reaction
Protein isoforms
Ribonucleic acid
Ribonucleoproteins, Small Nuclear - genetics
Ribonucleoproteins, Small Nuclear - physiology
RNA
RNA Splicing Factors
RNA-Binding Proteins
Site Selection
Spliceosomes - metabolism
Splicing
Transcripts (Written Records)
Transfection
Tumor Cells, Cultured
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Summary:Alu repetitive elements can be inserted into mature messenger RNAs via a splicing-mediated process termed exonization. To understand the molecular basis and the regulation of the process of turning intronic Alus into new exons, we compiled and analyzed a data set of human exonized Alus. We revealed a mechanism that governs 3' splice-site selection in these exons during alternative splicing. On the basis of these findings, we identified mutations that activated the exonization of a silent intronic Alu.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1082588