Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry

The annotated genomes of organisms define a 'blueprint' of their possible gene products. Post-genome analyses attempt to confirm and modify the annotation and impose a sense of the spatial, temporal and developmental usage of genetic information by the organism. Here we describe a large-sc...

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Bibliographic Details
Published in:Nature (London) 2002-10, Vol.419 (6906), p.537-542
Main Authors: Stunnenberg, Hendrik G, Mann, Matthias, Lasonder, Edwin, Ishihama, Yasushi, Andersen, Jens S, Vermunt, Adriaan M. W, Pain, Arnab, Sauerwein, Robert W, Eling, Wijnand M. C, Hall, Neil, Waters, Andrew P
Format: Article
Language:English
Subjects:
Animals
Erythrocytes - parasitology
Female
Freshwater
Genomics
Germ Cells
Humans
Life Cycle Stages
Malaria
Male
Mass Spectrometry - methods
Parasites
Plasmodium falciparum - chemistry
Plasmodium falciparum - genetics
Plasmodium falciparum - growth & development
Proteins
Proteome
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Scientific imaging
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Summary:The annotated genomes of organisms define a 'blueprint' of their possible gene products. Post-genome analyses attempt to confirm and modify the annotation and impose a sense of the spatial, temporal and developmental usage of genetic information by the organism. Here we describe a large-scale, high-accuracy (average deviation less than 0.02 Da at 1,000 Da) mass spectrometric proteome analysis of selected stages of the human malaria parasite Plasmodium falciparum. The analysis revealed 1,289 proteins of which 714 proteins were identified in asexual blood stages, 931 in gametocytes and 645 in gametes. The last two groups provide insights into the biology of the sexual stages of the parasite, and include conserved, stage-specific, secreted and membrane-associated proteins. A subset of these proteins contain domains that indicate a role in cell-cell interactions, and therefore can be evaluated as potential components of a malaria vaccine formulation. We also report a set of peptides with significant matches in the parasite genome but not in the protein set predicted by computational methods.
ISSN:0028-0836
1476-4687
DOI:10.1038/nature01111