Development of microsatellite markers from tartary buckwheat

A genomic library enriched with (gT)n repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5'-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and...

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Bibliographic Details
Published in:Biotechnology letters 2007-05, Vol.29 (5), p.823-827
Main Authors: Li, Yan-Qin, Shi, Tong-Lin, Zhang, Zong-Wen
Format: Article
Language:English
Subjects:
Anchored PCR
Animal populations
Biological and medical sciences
Biotechnology
Degenerate primer
DNA Primers - metabolism
Fagopyrum - genetics
Fagopyrum tataricum
Fundamental and applied biological sciences. Psychology
microsatellite repeats
Microsatellite Repeats - genetics
Plants diversity
Polymerase Chain Reaction
Polymorphism, Genetic
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Summary:A genomic library enriched with (gT)n repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5'-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed that the degenerate primer was reliably anchoring at the 5'-end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of tartary buckwheat.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-006-9293-2