Clonogenic assay and in vitro chemosensitivity testing of human urologic malignancies

Over the past year, we have attempted to grow both primary and metastatic urologic malignancies using a recently developed human tumor cloning system. Formation of colonies in vitro occurred in 125 of 164 primary tumors (76%), including 34 of 47 uroepithelial cancer specimens, 45 of 50 renal cell ca...

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Bibliographic Details
Published in:Cancer 1982-10, Vol.50 (7), p.1332-1338
Main Authors: Sarosdy, Michael F., Lamm, Donald L., Radwin, Howard M., Von Hoff, Daniel D.
Format: Article
Language:English
Subjects:
Acid Phosphatase - analysis
Agar
alpha-Fetoproteins - analysis
Antineoplastic Agents - pharmacology
Cell Count
Chorionic Gonadotropin - analysis
Chorionic Gonadotropin, beta Subunit, Human
Clone Cells
Cytological Techniques
Drug Resistance
Genital Neoplasms, Male - metabolism
Genital Neoplasms, Male - pathology
Genital Neoplasms, Male - ultrastructure
Humans
Male
Microscopy, Electron
Neoplasm Metastasis
Neoplasms - pathology
Peptide Fragments - analysis
Urologic Neoplasms - metabolism
Urologic Neoplasms - pathology
Urologic Neoplasms - ultrastructure
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Summary:Over the past year, we have attempted to grow both primary and metastatic urologic malignancies using a recently developed human tumor cloning system. Formation of colonies in vitro occurred in 125 of 164 primary tumors (76%), including 34 of 47 uroepithelial cancer specimens, 45 of 50 renal cell cancer specimens, 24 of 33 prostatic cancer specimens, and 22 of 34 testicular cancer specimens. A large percentage of metastatic cancers have also been successfully cultured. Growth sufficient for chemosensitivity testing ranged from 43% of the uroepithelial cancers cultured to 64% of the renal cell cancer specimens cultured. When in vitro chemosensitivity testing was performed, the in vitro chemosensitivity results show a striking similarity to clinical response rates for the same agents used for these tumors. Overall the human tumor cloning system appears to be a reasonable model for the study of human urologic malignancies.
ISSN:0008-543X
1097-0142
DOI:10.1002/1097-0142(19821001)50:7<1332::AID-CNCR2820500719>3.0.CO;2-8