Persistent Expression of Fas/FasL mRNA in the Mouse Hippocampus After a Single NMDA Injection

: Synaptic reorganization plays a very important role in brain adaptations to environmental stimuli, diseases, and aging processes. The NMDA model of excitotoxic injury was used to investigate the long‐term molecular changes in the surviving neural cells in the mouse hippocampus. We demonstrated tha...

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Bibliographic Details
Published in:Journal of neurochemistry 1998-10, Vol.71 (4), p.1773-1776
Main Authors: Shin, Song‐Woo, Park, Jong‐Wook, Suh, Min‐Ho, Suh, Sung‐Il, Choe, Byung‐Kil
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface
Apoptosis
Biological and medical sciences
Cells, Cultured
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Fas Ligand Protein
fas Receptor - biosynthesis
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
Hippocampus
Hippocampus - drug effects
Hippocampus - metabolism
Injections, Intraperitoneal
Ligands
Long‐term gene expression
Membrane Glycoproteins - biosynthesis
Mice
Mice, Inbred ICR
N-Methylaspartate - administration & dosage
NMDA
Rats
Rats, Sprague-Dawley
RNA, Messenger - biosynthesis
RNA, Messenger - drug effects
Tumor Cells, Cultured
Vertebrates: nervous system and sense organs
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Summary:: Synaptic reorganization plays a very important role in brain adaptations to environmental stimuli, diseases, and aging processes. The NMDA model of excitotoxic injury was used to investigate the long‐term molecular changes in the surviving neural cells in the mouse hippocampus. We demonstrated that a single intraperitoneal injection of NMDA produces persistent expression of c‐fos, c‐jun, Fas, and Fas ligand (FasL) mRNA in the hippocampus for 5 months. To determine the cellular origin of those gene transcripts in our in vivo model, a glial cell line and primary fetal neuronal culture were used to investigate the inducibility of the c‐fos, c‐jun, Fas, and FasL mRNA by NMDA. Both c‐fos and Fas mRNA expression was observed in the NMDA‐treated glial or neuronal cultures; however, c‐jun and FasL mRNA was undetectable in this study. In our in vivo model, mossy fiber sprouting and apoptosis were also observed up to 40 days after the NMDA injection. Therefore, we hypothesize that the observed long‐term expression of c‐fos, c‐jun, Fas, and FasL mRNAs may reflect the ongoing synaptic reorganization.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.1998.71041773.x