SAP30, a Component of the mSin3 Corepressor Complex Involved in N-CoR-Mediated Repression by Specific Transcription Factors

The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and i...

Full description

Saved in:
Bibliographic Details
Published in:Molecular cell 1998-07, Vol.2 (1), p.33-42
Main Authors: Laherty, Carol D., Billin, Andrew N., Lavinsky, Robert M., Yochum, Gregory S., Bush, Angela C., Sun, Jian-Min, Mullen, Tina-Marie, Davie, James R., Rose, David W., Glass, Christopher K., Rosenfeld, Michael G., Ayer, Donald E., Eisenman, Robert N.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cell Line, Transformed
DNA-Binding Proteins - physiology
Histone Deacetylases - genetics
Histone Deacetylases - physiology
Homeodomain Proteins - physiology
Humans
Kidney
Macromolecular Substances
Mice
Models, Genetic
Molecular Sequence Data
Multiprotein Complexes
Nuclear Proteins - physiology
Nuclear Receptor Co-Repressor 1
Receptors, Estrogen - antagonists & inhibitors
Receptors, Retinoic Acid - physiology
Receptors, Thyroid Hormone - physiology
Repressor Proteins - physiology
Saccharomyces cerevisiae Proteins
Transcription Factor Pit-1
Transcription Factors - physiology
Transcription, Genetic
Transfection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3–histone deacetylase complex to a specific subset of N-CoR corepressor complexes.
ISSN:1097-2765
1097-4164
1097-4164
DOI:10.1016/S1097-2765(00)80111-2