Isolation and partial characterization of antifungal metabolites of Bacillus pumilus

Antifungal metabolites produced by Bacillus pumilus in Potato Dextrose Broth (PDB) were isolated from culture supernatant fluid by precipitation with ammonium sulphate. The antifungal metabolites inhibited mycelial growth of many species of Aspergillus, Penicillium and Fusarium. They also inhibited...

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Bibliographic Details
Published in:Journal of applied microbiology 1998-06, Vol.84 (6), p.959-968
Main Authors: Munimbazi, C, Bullerman, L.B
Format: Article
Language:English
Subjects:
Adolescent
Animals
Antibiosis
Antifungal Agents - biosynthesis
Antifungal Agents - isolation & purification
Antifungal Agents - pharmacology
Aspergillus
Bacillus - growth & development
Bacillus - metabolism
Bacillus pumilus
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Chromatography, Thin Layer
Culture Media
Detergents
food contamination
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
Fungi - drug effects
Fungi - growth & development
Fungi - metabolism
Fusarium
Hot Temperature
Humans
Hydrogen-Ion Concentration
Hydrolysis
Miscellaneous
Mission oriented research
Penicillium
Peptide Hydrolases - metabolism
plant health
plant pathogenic fungi
plant products
Solubility
stored product protection
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Summary:Antifungal metabolites produced by Bacillus pumilus in Potato Dextrose Broth (PDB) were isolated from culture supernatant fluid by precipitation with ammonium sulphate. The antifungal metabolites inhibited mycelial growth of many species of Aspergillus, Penicillium and Fusarium. They also inhibited production of aflatoxins, cyclopiazonic acid, ochratoxin A and patulin. The metabolites were heat-stable and remained active after sterilization at 121 degrees C for 15 min. Their activity was stable over a wide range of pH (2-10). The metabolites were resistant to hydrolysis by various proteases, peptidases and other enzymes. They were also resistant to denaturation by many protein-denaturing detergents except Nonidet P-40. The metabolites were soluble in water and relatively polar organic solvents. Chromatographic bioassay revealed that a crude precipitate of the metabolites contained only one compound with antifungal activity. The active compound did not form a fluorescent derivative with fluorescamine suggesting that the compound is either a cyclic polypeptide or a non-peptidic compound.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1998.00431.x