The Meiosis-Specific Hop2 Protein of S. cerevisiae Ensures Synapsis between Homologous Chromosomes

The hop2 mutant of S. cerevisiae displays a novel phenotype: meiotic chromosomes form nearly wild-type amounts of synaptonemal complex, but most chromosomes are engaged in synapsis with nonhomologous partners. The meiosis-specific Hop2 protein localizes to chromosomes prior to and during synapsis an...

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Bibliographic Details
Published in:Cell 1998-08, Vol.94 (3), p.375-386
Main Authors: Leu, Jun-Yi, Chua, Penelope R, Roeder, G.Shirleen
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Cell Cycle Proteins
Chromosomes - genetics
Cloning, Molecular
DNA Damage
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - isolation & purification
Fungal Proteins - physiology
Gene Conversion
Gene Expression Regulation, Fungal
Meiosis - genetics
Molecular Sequence Data
Mutation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
Synaptonemal Complex - genetics
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Summary:The hop2 mutant of S. cerevisiae displays a novel phenotype: meiotic chromosomes form nearly wild-type amounts of synaptonemal complex, but most chromosomes are engaged in synapsis with nonhomologous partners. The meiosis-specific Hop2 protein localizes to chromosomes prior to and during synapsis and in the absence of the double-strand breaks that initiate recombination. hop2 strains sustain a wild-type level of meiotic double-strand breaks, but these breaks remain unrepaired. The hop2 mutant arrests at the pachytene stage of meiotic prophase with the RecA-like protein Dmc1 located at numerous sites along synapsed chromosomes. We propose that the Hop2 protein functions to prevent synapsis between nonhomologous chromosomes .
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)81480-4