Promyelocytic leukaemia zinc finger protein (PLZF) is a glucocorticoid‐ and progesterone‐induced transcription factor in human endometrial stromal cells and myometrial smooth muscle cells

The promyelocytic leukaemia zinc finger (PLZF) protein belongs to the family of Krüppel‐like zinc finger proteins. It is a transcriptional repressor involved in cell cycle control and has been implicated in limb development, differentiation of myeloid cells, and spermatogenesis. Little is known abo...

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Bibliographic Details
Published in:Molecular human reproduction 2003-10, Vol.9 (10), p.611-623
Main Authors: Fahnenstich, Jasmin, Nandy, Andreas, Milde‐Langosch, Karin, Schneider‐Merck, Tanja, Walther, Norbert, Gellersen, Birgit
Format: Article
Language:English
Subjects:
Base Sequence
Cells, Cultured
Dexamethasone - pharmacology
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Endometrium - cytology
Female
Glucocorticoids - pharmacology
Humans
Key words: endometrium/glucocorticoid/myometrium/PLZF/progesterone
Kruppel-Like Transcription Factors
Molecular Sequence Data
Myocytes, Smooth Muscle - drug effects
Myocytes, Smooth Muscle - metabolism
Myometrium - cytology
Progesterone - pharmacology
Promoter Regions, Genetic - genetics
Promyelocytic Leukemia Zinc Finger Protein
Receptors, Glucocorticoid - metabolism
Receptors, Progesterone - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Alignment
Signal Transduction - drug effects
Stromal Cells - drug effects
Stromal Cells - metabolism
Transcription Factor AP-1 - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription Initiation Site
Tretinoin - pharmacology
Up-Regulation - drug effects
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Summary:The promyelocytic leukaemia zinc finger (PLZF) protein belongs to the family of Krüppel‐like zinc finger proteins. It is a transcriptional repressor involved in cell cycle control and has been implicated in limb development, differentiation of myeloid cells, and spermatogenesis. Little is known about the regulation of PLZF expression. In search for mediators of progesterone signalling in the female reproductive tract, we discovered induction of PLZF mRNA in primary cultures of human endometrial stromal cells and myometrial smooth muscle cells (SMC) in response to progesterone. Surprisingly, dexamethasone was a more potent inducer of PLZF expression than progesterone and elicited a sustained up‐regulation of PLZF mRNA levels within 2 h. Immunofluorescence showed localization of PLZF to the nuclei of dexamethasone‐treated SMC. In uterine biopsies, nuclear staining for PLZF was found in myometrial cells and endometrial stromal cells of the secretory phase. The transcriptional start site of the PLZF gene was located to position –5801 in SMC. Transfected promoter constructs containing up to 4.1 kb of 5′‐flanking DNA were not induced by activated glucocorticoid or progesterone receptor. In contrast, co‐transfection of c‐jun and c‐fos expression vectors resulted in stimulation of reporter gene activity, indicating an involvement of AP‐1 transcription factors in PLZF expression.
ISSN:1360-9947
1460-2407
1460-2407
DOI:10.1093/molehr/gag080