Application of Avidin−Biotin Technology and Adsorptive Transfer Stripping Square-Wave Voltammetry for Detection of DNA Hybridization and Avidin in Transgenic Avidin Maize

The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgeni...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2003-06, Vol.75 (11), p.2663-2669
Main Authors: Masarik, Michal, Kizek, Rene, Kramer, Karl J, Billova, Sabina, Brazdova, Marie, Vacek, Jan, Bailey, Michele, Jelen, Frantisek, Howard, John A
Format: Article
Language:English
Subjects:
Adsorption
adsorptive transfer stripping square-wave voltammetry
analytical methods
avidin
Avidin - analysis
Avidin - chemistry
Avidin - genetics
bacterial proteins
Biological and medical sciences
biotin
Biotin - chemistry
chickens
corn
Diverse techniques
DNA
DNA - metabolism
Electrochemistry - methods
Electrodes
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
nucleic acid hybridization
Nucleic Acid Hybridization - methods
oligonucleotides
Plants, Genetically Modified - chemistry
Plants, Genetically Modified - genetics
quantitative analysis
recombinant proteins
streptavidin
Streptavidin - analysis
Streptavidin - chemistry
transgenes
transgenic plants
Zea mays
Zea mays - chemistry
Zea mays - genetics
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Summary:The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/ac020788z