Technetium-99m scintigraphy to visualize T-cell homing in vivo: a preclinical study

The knowledge of lymphocyte distribution is very usefulness in monitoring therapeutic treatments. We present here a method employed in clinical practice, the scintigraphy, to study in the rat the physiologic lymphocyte traffic. Rat T cells labeled with 99mTc were injected in syngeneic animals, and t...

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Bibliographic Details
Published in:Nuclear medicine and biology 2003-08, Vol.30 (6), p.633-642
Main Authors: Fulgenzi, Alessandro, Ferrero, Elisabetta, Gasparini, Massimo, Casati, Rosangela, Riccardo Colombo, Fabio, Gerundini, Paolo, Elena Ferrero, Maria
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
CD4 Lymphocyte Count - methods
CD4-Positive T-Lymphocytes - diagnostic imaging
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - physiology
Cell homing
Cell Movement - immunology
Cell Movement - physiology
Lymphoid Tissue - diagnostic imaging
Lymphoid Tissue - immunology
Male
Medical sciences
Memory cells
Naïve cells
Organ Specificity
Radionuclide Imaging
Radiopharmaceuticals
Rat
Rats
Rats, Inbred F344
Scintigraphy
T lymphocytes
T-Lymphocytes - diagnostic imaging
T-Lymphocytes - immunology
T-Lymphocytes - physiology
Technetium Tc 99m Exametazime
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Summary:The knowledge of lymphocyte distribution is very usefulness in monitoring therapeutic treatments. We present here a method employed in clinical practice, the scintigraphy, to study in the rat the physiologic lymphocyte traffic. Rat T cells labeled with 99mTc were injected in syngeneic animals, and their fate was studied by serial scintigraphic scanning. Sorted naïve CD4 + CD45RC bright T cells homed to lymphoid organs and accumulated in spleen. CD4 + CD45RC dim memory lymphocytes first reached the liver and the lungs and recirculated. The results obtained by using the scintigraphic method to in vivo study the lymphocyte homing in rats are comparable to those obtained with previously used experimental methods. We consider the scintigraphic method a useful tool to in vivo track lymphocytes and to address therapeutic treatment in men.
ISSN:0969-8051
1872-9614
DOI:10.1016/S0969-8051(03)00051-9