Hypophysiotropic activity of histone H3 in vitro

To assess the effect of histone H3 on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30 microM) stimulated t...

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Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2003-05, Vol.24 (5), p.671-678
Main Authors: Brown, Oscar A, Sosa, Yolanda E, Naumovich, Diana, Hereñú, Claudia B, Goya, Rodolfo G
Format: Article
Language:English
Subjects:
Animals
Dose-Response Relationship, Drug
Female
Histones - agonists
Histones - metabolism
Histones - pharmacology
Hypothalamo-Hypophyseal System
Peptides - metabolism
Pituitary Gland, Anterior - drug effects
Pituitary Gland, Anterior - metabolism
Rats
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled - agonists
Receptors, Ghrelin
Signal Transduction
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Summary:To assess the effect of histone H3 on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30 microM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6 microM H3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals.
ISSN:0196-9781
1873-5169