Transcriptional and post-translational regulation of S-adenosyl-L-methionine : salicylic acid carboxyl methyltransferase (SAMT) during Stephanotis floribunda flower development

Methyl salicylate (MeSA) and a number of other volatiles are primarily emitted in the evening/night by Stephanotis floribunda leading to attraction of night active pollinators. A second minor emission peak for MeSA occurs in the morning/day. To understand these emission patterns, we have studied in...

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Bibliographic Details
Published in:Journal of plant physiology 2003-06, Vol.160 (6), p.635-643
Main Authors: Pott, Marcella B., Effmert, U.t.a., Piechulla, Birgit
Format: Article
Language:English
Subjects:
Apocynaceae - enzymology
Apocynaceae - genetics
Apocynaceae - growth & development
Biological and medical sciences
Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation
circadian clock
Circadian Rhythm - physiology
Flowers - enzymology
Flowers - genetics
Flowers - growth & development
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental - radiation effects
Gene Expression Regulation, Enzymologic - radiation effects
Gene Expression Regulation, Plant - radiation effects
Light
Methyltransferases - genetics
Methyltransferases - metabolism
Plant physiology and development
Plant Proteins - genetics
Plant Proteins - metabolism
Protein Processing, Post-Translational - radiation effects
RNA, Messenger - genetics
RNA, Messenger - metabolism
S-adenosyl-L-methionine : salicylic acid carboxyl methyltransferase (SAMT)
Salicylates - metabolism
Salicylic Acid - metabolism
Stephanotis floribunda
Temperature
Transcription, Genetic - radiation effects
transcriptional and post-translational regulation
Vegetative and sexual reproduction, floral biology, fructification
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Summary:Methyl salicylate (MeSA) and a number of other volatiles are primarily emitted in the evening/night by Stephanotis floribunda leading to attraction of night active pollinators. A second minor emission peak for MeSA occurs in the morning/day. To understand these emission patterns, we have studied in detail the temporal regulation of the last step of the biosynthetic pathway of MeSA, the convertion of salicylic acid (SA) to MeSA catalysed by S-adenosyl-L-methionine : salicylic acid carboxyl methyltransferase (SAMT). We observed that in young flowers a maximum in SAMT activity occurs in the night, and that in flowers which were open longer than 4 days, two SAMT activity maxima occurred per day. These maxima correlated well with dawn and dusk and the previously detected MeSA emission peaks. The SAMT mRNA levels, however, have a broad maximum during the dark phase, while the SAMT protein levels continuously increase during floral development without showing daily rhythms. Furthermore, under continuous illumination (LL) the SAMT mRNA levels and activity patterns oscillate, suggesting the involvement of a circadian clock in the regulation network. Taken together, this analysis clearly demonstrates that regulation of MeSA emission occurs both at the transcriptional and post-translational levels, indicating that control at more than one level is necessary to guarantee the precise timing of volatile emission in flowers of S. floribunda
ISSN:0176-1617
1618-1328
DOI:10.1078/0176-1617-00968