High-content assays for ligand regulation of G-protein-coupled receptors

High-content assays rely on the imaging of cellular events. They can be used to monitor the activation of G-protein-coupled receptors (or other receptors), their internalization into the cell, or alterations in their amount. In addition, multiplexed assays can provide further information about the c...

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Bibliographic Details
Published in:Drug Discovery Today 2003-07, Vol.8 (13), p.579-585
Main Author: Milligan, Graeme
Format: Article
Language:English
Subjects:
Animals
beta-arrestins
G-protein-coupled receptors
Genome, Human
green fluorescent protein
GTP-Binding Protein Regulators - drug effects
GTP-Binding Protein Regulators - metabolism
Humans
Ligands
multiplexed assays
protein translocation
Receptors, Cell Surface - drug effects
Receptors, Cell Surface - metabolism
Technology, Pharmaceutical - trends
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Summary:High-content assays rely on the imaging of cellular events. They can be used to monitor the activation of G-protein-coupled receptors (or other receptors), their internalization into the cell, or alterations in their amount. In addition, multiplexed assays can provide further information about the characteristics of the receptor. Recent improvements in throughput using high-content screening platforms means that such assays are now an integral element of functional analysis in the drug discovery process. High-content assays rely on the imaging of cellular events. Recent improvements in throughput using high-content screening platforms means that such assays are now an integral element of functional analysis in the drug discovery process.
ISSN:1359-6446
1878-5832
DOI:10.1016/S1359-6446(03)02738-7