Species Dependency of In Vitro Macrophage Activation by Bacterial Peptidoglycans

The effect of various bacterial cell wall components on in vitro biological function of murine peritoneal exudate macrophages was evaluated. We examined four different parameters of metabolic activity and monokine secretion. Peritoneal exudate macrophages from rats and guinea pigs, all of the strain...

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Published in:MICROBIOLOGY and IMMUNOLOGY 1992, Vol.36(11), pp.1155-1171
Main Authors: Nagao, Shigeki, Akagawa, Kiyoko S., Okada, Fumito, Harada, Yuji, Yagawa, Katsuro, Kato, Keijiro, Tanigawa, Yoshinori
Format: Article
Language:English
Subjects:
Acetylmuramyl-Alanyl-Isoglutamine - analogs & derivatives
Acetylmuramyl-Alanyl-Isoglutamine - pharmacology
Animals
Bacteria
Bacteriology
Biological and medical sciences
Cell Wall - chemistry
Cell Wall - immunology
Cytotoxicity, Immunologic
Female
Fundamental and applied biological sciences. Psychology
Glucosamine - metabolism
Gram-Positive Bacteria - immunology
Gram-Positive Bacteria - ultrastructure
Guinea Pigs
In Vitro Techniques
Interleukin-1 - biosynthesis
Lipopolysaccharides - pharmacology
Macrophage Activation - drug effects
Macrophage Activation - immunology
Macrophages - drug effects
Macrophages - immunology
Mice
Mice, Inbred Strains
Microbiology
Morphology, structure, chemical composition
Peptidoglycan - pharmacology
Rats
Rats, Inbred Strains
Rodentia
Species Specificity
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:The effect of various bacterial cell wall components on in vitro biological function of murine peritoneal exudate macrophages was evaluated. We examined four different parameters of metabolic activity and monokine secretion. Peritoneal exudate macrophages from rats and guinea pigs, all of the strains tested, were stimulated by whole bacterial cell wall preparations, purified bacterial cell wall peptido-glucans, its water-soluble peptidoglycan fragments, muramyl dipeptides and amphipathic substances. Murine peritoneal exudate macrophages were activated by amphipathic substances of gram-positive bacteria. However, macrophages from mice, irrespective of strains, were not stimulated in the in vitro assay systems by purified bacterial cell wall peptidoglycan, water-soluble bacterial peptidoglycan fragments or muramyl dipeptides. These results suggest that macrophage activation by bacterial peptidoglycan in vitro is animal species specific.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1992.tb02119.x