The nitric oxide donor molsidomine prevents ischemia/reperfusion injury of the adult rat small intestine

It is suggested that gastrointestinal mucosal blood flow depends on a balanced release of vasoactive substances from the endothelium. The present study investigated the effects of molsidomine on the small intestine after ischemia-reperfusion (I/R) injury in four groups of 10 rats each composed: (1)...

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Published in:Pediatric surgery international 2003-06, Vol.19 (4), p.305-308
Main Authors: ÖZTÜRK, Hayrettin, ALDEMIR, Mustafa, ALI IHSAN DOKUCU, YAGMUR, Yusuf, KILINC, Nihal, AHMET HIKMET SAHIN
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Gastroenterology. Liver. Pancreas. Abdomen
Intestinal Mucosa - pathology
Intestine, Small - blood supply
Intestine, Small - physiology
Lipid Peroxidation
Malondialdehyde - analysis
Medical sciences
Molsidomine - pharmacology
Molsidomine - therapeutic use
Other diseases. Semiology
Rats
Rats, Sprague-Dawley
Regional Blood Flow - drug effects
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Vasodilator Agents - pharmacology
Vasodilator Agents - therapeutic use
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Summary:It is suggested that gastrointestinal mucosal blood flow depends on a balanced release of vasoactive substances from the endothelium. The present study investigated the effects of molsidomine on the small intestine after ischemia-reperfusion (I/R) injury in four groups of 10 rats each composed: (1) SO, sham operation; (2) untreated I/R; (3) ML, I/R plus molsidomine pretreatment; (4) L-NAME, I/R plus N-omega-nitro-L-arginine methyl ester pretreatment. Intestinal ischemia for 45 min and reperfusion for 60 min were applied. Ileum specimens were obtained to determine the tissue level of malondialdehyde (MDA) and histologic changes. Mean MDA levels in the SO, untreated I/R, ML, and L-NAME groups were 95.60 +/- 2.59, 136.90 +/- 4.35, 121.10 +/- 3.38, and 137.40 +/- 4.42 nmol/g wet tissue, respectively. Although the MDA level in the ML group was higher than in the SO group ( P < 0.0001), it was significantly lower compared to the untreated I/R and L-NAME groups ( P < 0.0001, P < 0.0001). Mucosal injury scores (MIS) in groups 1-4 were 0.2 +/- 0.42, 3.9 +/- 0.73, 1.5 +/- 0.70, and 4.1 +/- 0.56, respectively. In group 3 the MIS was significantly lower than in groups 2 and 4 ( P < 0.0001, P < 0.0001). Molsidomine plays a role in attenuating reperfusion injury of the small intestine by depression of tissue MDA levels and MIS and regulates post-ischemic intestinal perfusion while restoring the intestinal microcirculatory blood flow and histologic injury.
ISSN:0179-0358
1437-9813
DOI:10.1007/s00383-002-0746-y