Characterization of novel polyomaviruses from Bornean and Sumatran orang-utans

1 Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands 2 Institute of Biotechnology, Vilnius, Lithuania 3 School of Veterinary and Biomedical Sciences, Murdoch University, Perth, Western Australia, Australia Correspondence Ernst J. Verschoor verschoor{at}bprc.nl Sero...

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Bibliographic Details
Published in:Journal of general virology 2010-03, Vol.91 (3), p.653-658
Main Authors: Groenewoud, Marlous J, Fagrouch, Zahra, van Gessel, Sabine, Niphuis, Henk, Bulavaite, Aiste, Warren, Kristin S, Heeney, Jonathan L, Verschoor, Ernst J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Ape Diseases - virology
Biological and medical sciences
Borneo
Cluster Analysis
DNA, Viral - chemistry
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Gene Order
Genes, Viral
Indonesia
Microbiology
Miscellaneous
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction - methods
Polyomavirus - classification
Polyomavirus - genetics
Polyomavirus - isolation & purification
Polyomavirus Infections - veterinary
Polyomavirus Infections - virology
Pongo abelii - virology
Pongo pygmaeus - virology
Sequence Alignment
Sequence Analysis, DNA
Synteny
Tumor Virus Infections - veterinary
Tumor Virus Infections - virology
Virology
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Summary:1 Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands 2 Institute of Biotechnology, Vilnius, Lithuania 3 School of Veterinary and Biomedical Sciences, Murdoch University, Perth, Western Australia, Australia Correspondence Ernst J. Verschoor verschoor{at}bprc.nl Serological screening of sera from orang-utans demonstrated a high percentage of sera that cross-reacted with antigens of the polyomavirus (PyV) simian virus 40. Analysis of archival DNA samples from 71 Bornean and eight Sumatran orang-utans with a broad-spectrum PCR assay resulted in the detection of PyV infections in 11 animals from both species. Sequence analysis of the amplicons revealed considerable differences between the PyVs from Bornean and Sumatran orang-utans. The genome from two PyVs, one from each species, was therefore amplified and sequenced. Both viral genomes revealed a characteristic PyV architecture, but lacked an obvious agnogene. Neighbour-joining analysis positioned the viruses in a large cluster together with viruses from bats, bovines, rodents and several primate PyVs from chimpanzees, African green monkeys, squirrel monkeys and the human Merkel cell PyV. Present address: Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Center, Utrecht, The Netherlands. Present address: Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. Present address: Department of Veterinary Medicine, University of Cambridge, Cambridge, UK. The GenBank/EMBL/DDBJ accession numbers for the VP1 sequences determined in this study are FN356900 [GenBank] –FN356910. Three supplementary figures showing additional sequence comparison data are available with the online version of this paper.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.017673-0