Spin labeling EPR

Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-dir...

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Bibliographic Details
Published in:Photosynthesis research 2009-12, Vol.102 (2-3), p.377-390
Main Authors: Klare, Johann P, Steinhoff, Heinz-Jürgen
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Electron Spin Resonance Spectroscopy - methods
Flowers & plants
Life Sciences
Membrane Proteins - chemistry
Models, Molecular
Photosynthesis
Plant Genetics and Genomics
Plant Physiology
Plant Sciences
Protein Conformation
Review
Rhodobacter sphaeroides
Spectrum analysis
Spin Labels
Time Factors
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Summary:Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling. Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the sensory rhodopsin-transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis and the photosynthetic reaction center from Rhodobacter sphaeroides R26.
ISSN:0166-8595
1573-5079
DOI:10.1007/s11120-009-9490-7