Protective effect of non-mitogenic human acidic fibroblast growth factor on hepatocyte injury

Aim:  To study whether non‐mitogenic human acidic fibroblast growth factor (nm‐haFGF) has protective effects on H2O2‐induced hepatocyte injury in vitro and CCl4‐induced hepatocyte injury in vivo. Methods:  (i) HL‐7702 hepatocytes were incubated with different concentrations of nm‐haFGF for 12 h, and...

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Published in:Hepatology research 2007-10, Vol.37 (10), p.836-844
Main Authors: Xu, Hua, Hai, Guang-fan, Xiang, Ji-zhou, Yao, Cheng-can, Zheng, Qing, Zhang, Qi-hao, Hong, Hai
Format: Article
Language:English
Subjects:
CCl4
H2O2
hepatic protection
hepatocyte
non-mitogenic human acidic fibroblast growth factor
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Summary:Aim:  To study whether non‐mitogenic human acidic fibroblast growth factor (nm‐haFGF) has protective effects on H2O2‐induced hepatocyte injury in vitro and CCl4‐induced hepatocyte injury in vivo. Methods:  (i) HL‐7702 hepatocytes were incubated with different concentrations of nm‐haFGF for 12 h, and then the activity of lactate dehydrogenase (LDH) in culture medium was detected, and genomic DNA electrophoresis analysis was observed after being exposed to H2O2 (8 mmol/L) for 4 h. Proximately, apoptotic rates and protein expressions of Bcl‐2 and Bax of HL‐7702 cell were detected after being exposed to H2O2 (0.2 mmol/L) for 20 h. (ii) Being injected intraperitoneally with nm‐haFGF, mice were treated with CCl4 intraperitoneally to induce hepatic injury. Twenty‐four hours later, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured and histopathologic changes were evaluated. Results:  (i) In vitro tests: LDH activities and apoptotic rates decreased, the protein expression of Bcl‐2 increased and Baxdecreased in nm‐haFGF‐treated groups at the concentrations of 100 150 and 200 ng/mL, compared with that in the model control group, which was treated with H2O2 alone. The genomic DNA remained nearly intact at the concentrations of 150 and 200 ng/mL. (ii) In vivo tests: serum ALT and AST in nm‐haFGF‐treated groups (10 μg/kg and 20 μg/kg) were much lower as compared to the model control group, which was treated with CCl4 alone. Histological examination showed that nm‐haFGF markedly ameliorated hepatocytes vacuolation, cloudy swelling and inflammatory cells infiltration induced by CCl4. Conclusion:  nm‐haFGF had protective effects against H2O2‐induced hepatocyte injury in vitro and CCl4‐induced acute liver injury in vivo.
ISSN:1386-6346
1872-034X
DOI:10.1111/j.1872-034X.2007.00131.x